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Quantification of total and phosphorylated STAT3 by calibrated western blotting
Quantification of intracellular proteins is essential to understand signaling. Here, we describe quantification of the expression and phosphorylation of the transcription factor STAT3. We present isolation of total and phosphorylated STAT3 from cell lysates by immunoprecipitation, followed by SDS-PA...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485629/ https://www.ncbi.nlm.nih.gov/pubmed/37669163 http://dx.doi.org/10.1016/j.xpro.2023.102508 |