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A hybrid RNA FISH immunofluorescence protocol on Drosophila polytene chromosomes

OBJECTIVES: Investigating protein-DNA interactions is imperative to understanding fundamental concepts such as cell growth, differentiation, and cell development in many systems. Sequencing techniques such as ChIP-seq can yield genome-wide DNA binding profiles of transcription factors; however this...

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Detalles Bibliográficos
Autores principales: Gilbonio, Hannah E., Puckett, Gwyn L., Nguyen, Erica, Rieder, Leila E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486132/
https://www.ncbi.nlm.nih.gov/pubmed/37679799
http://dx.doi.org/10.1186/s13104-023-06482-0