PCR-Based Strategy for Introducing CRISPR/Cas9 Machinery into Hematopoietic Cell Lines

SIMPLE SUMMARY: We used PCR-generated small CRISPR constructs to edit two genes (IDH2 and MYBL2) in hard-to-transfect hemopoietic cells, which are central to the progression of the devastating disease known as acute myeloid leukemia LMA (AML). MYBL2 is a transcription factor; when AML patients show...

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Detalles Bibliográficos
Autores principales: González-Romero, Elisa, Martínez-Valiente, Cristina, García-García, Gema, Rosal-Vela, Antonio, Millán, José María, Sanz, Miguel Ángel, Sanz, Guillermo, Liquori, Alessandro, Cervera, José Vicente, Vázquez-Manrique, Rafael P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487029/
https://www.ncbi.nlm.nih.gov/pubmed/37686539
http://dx.doi.org/10.3390/cancers15174263

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487029/
https://www.ncbi.nlm.nih.gov/pubmed/37686539
http://dx.doi.org/10.3390/cancers15174263

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