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The Correlation of In Vivo MR Spectroscopy and Ex Vivo 2-Hydroxyglutarate Concentration for the Prediction of Isocitrate Dehydrogenase Mutation Status in Diffuse Glioma
Isocitrate dehydrogenase (IDH) mutation status is an important biomarker in the glioma-defining subtype and corresponding prognosis. This study proposes a straightforward method for 2-hydroxyglutarate (2-HG) quantification by MR spectroscopy for IDH mutation status detection and directly compares in...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487112/ https://www.ncbi.nlm.nih.gov/pubmed/37685329 http://dx.doi.org/10.3390/diagnostics13172791 |
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author | van Dijken, Bart R. J. Jeltema, Hanne-Rinck Kłos, Justyna van Laar, Peter Jan Enting, Roelien H. Maatman, Ronald G. H. J. Bijsterveld, Klaas Den Dunnen, Wilfred F. A. Dierckx, Rudi A. Sijens, Paul E. van der Hoorn, Anouk |
author_facet | van Dijken, Bart R. J. Jeltema, Hanne-Rinck Kłos, Justyna van Laar, Peter Jan Enting, Roelien H. Maatman, Ronald G. H. J. Bijsterveld, Klaas Den Dunnen, Wilfred F. A. Dierckx, Rudi A. Sijens, Paul E. van der Hoorn, Anouk |
author_sort | van Dijken, Bart R. J. |
collection | PubMed |
description | Isocitrate dehydrogenase (IDH) mutation status is an important biomarker in the glioma-defining subtype and corresponding prognosis. This study proposes a straightforward method for 2-hydroxyglutarate (2-HG) quantification by MR spectroscopy for IDH mutation status detection and directly compares in vivo 2-HG MR spectroscopy with ex vivo 2-HG concentration measured in resected tumor tissue. Eleven patients with suspected lower-grade glioma (ten IDH1; one IDHwt) were prospectively included. Preoperatively, 3T point-resolved spectroscopy (PRESS) was acquired; 2-HG was measured as the percentage elevation of Glx3 (the sum of 2-HG and Glx) compared to Glx4. IDH mutation status was assessed by immunochemistry or direct sequencing. The ex vivo 2-HG concentration was determined in surgically obtained tissue specimens using gas chromatography–mass spectrometry. Pearson correlation was used for assessing the correlation between in vivo MR spectroscopy and ex vivo 2-HG concentration. MR spectroscopy was positive for 2-HG in eight patients, all of whom had IDH1 tumors. A strong correlation (r = 0.80, p = 0.003) between 2-HG MR spectroscopy and the ex vivo 2-HG concentration was found. This study shows in vivo 2-HG MR spectroscopy can non-invasively determine IDH status in glioma and demonstrates a strong correlation with ex vivo 2-HG concentration in patients with lower-grade glioma. |
format | Online Article Text |
id | pubmed-10487112 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104871122023-09-09 The Correlation of In Vivo MR Spectroscopy and Ex Vivo 2-Hydroxyglutarate Concentration for the Prediction of Isocitrate Dehydrogenase Mutation Status in Diffuse Glioma van Dijken, Bart R. J. Jeltema, Hanne-Rinck Kłos, Justyna van Laar, Peter Jan Enting, Roelien H. Maatman, Ronald G. H. J. Bijsterveld, Klaas Den Dunnen, Wilfred F. A. Dierckx, Rudi A. Sijens, Paul E. van der Hoorn, Anouk Diagnostics (Basel) Article Isocitrate dehydrogenase (IDH) mutation status is an important biomarker in the glioma-defining subtype and corresponding prognosis. This study proposes a straightforward method for 2-hydroxyglutarate (2-HG) quantification by MR spectroscopy for IDH mutation status detection and directly compares in vivo 2-HG MR spectroscopy with ex vivo 2-HG concentration measured in resected tumor tissue. Eleven patients with suspected lower-grade glioma (ten IDH1; one IDHwt) were prospectively included. Preoperatively, 3T point-resolved spectroscopy (PRESS) was acquired; 2-HG was measured as the percentage elevation of Glx3 (the sum of 2-HG and Glx) compared to Glx4. IDH mutation status was assessed by immunochemistry or direct sequencing. The ex vivo 2-HG concentration was determined in surgically obtained tissue specimens using gas chromatography–mass spectrometry. Pearson correlation was used for assessing the correlation between in vivo MR spectroscopy and ex vivo 2-HG concentration. MR spectroscopy was positive for 2-HG in eight patients, all of whom had IDH1 tumors. A strong correlation (r = 0.80, p = 0.003) between 2-HG MR spectroscopy and the ex vivo 2-HG concentration was found. This study shows in vivo 2-HG MR spectroscopy can non-invasively determine IDH status in glioma and demonstrates a strong correlation with ex vivo 2-HG concentration in patients with lower-grade glioma. MDPI 2023-08-29 /pmc/articles/PMC10487112/ /pubmed/37685329 http://dx.doi.org/10.3390/diagnostics13172791 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article van Dijken, Bart R. J. Jeltema, Hanne-Rinck Kłos, Justyna van Laar, Peter Jan Enting, Roelien H. Maatman, Ronald G. H. J. Bijsterveld, Klaas Den Dunnen, Wilfred F. A. Dierckx, Rudi A. Sijens, Paul E. van der Hoorn, Anouk The Correlation of In Vivo MR Spectroscopy and Ex Vivo 2-Hydroxyglutarate Concentration for the Prediction of Isocitrate Dehydrogenase Mutation Status in Diffuse Glioma |
title | The Correlation of In Vivo MR Spectroscopy and Ex Vivo 2-Hydroxyglutarate Concentration for the Prediction of Isocitrate Dehydrogenase Mutation Status in Diffuse Glioma |
title_full | The Correlation of In Vivo MR Spectroscopy and Ex Vivo 2-Hydroxyglutarate Concentration for the Prediction of Isocitrate Dehydrogenase Mutation Status in Diffuse Glioma |
title_fullStr | The Correlation of In Vivo MR Spectroscopy and Ex Vivo 2-Hydroxyglutarate Concentration for the Prediction of Isocitrate Dehydrogenase Mutation Status in Diffuse Glioma |
title_full_unstemmed | The Correlation of In Vivo MR Spectroscopy and Ex Vivo 2-Hydroxyglutarate Concentration for the Prediction of Isocitrate Dehydrogenase Mutation Status in Diffuse Glioma |
title_short | The Correlation of In Vivo MR Spectroscopy and Ex Vivo 2-Hydroxyglutarate Concentration for the Prediction of Isocitrate Dehydrogenase Mutation Status in Diffuse Glioma |
title_sort | correlation of in vivo mr spectroscopy and ex vivo 2-hydroxyglutarate concentration for the prediction of isocitrate dehydrogenase mutation status in diffuse glioma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487112/ https://www.ncbi.nlm.nih.gov/pubmed/37685329 http://dx.doi.org/10.3390/diagnostics13172791 |
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