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The Correlation of In Vivo MR Spectroscopy and Ex Vivo 2-Hydroxyglutarate Concentration for the Prediction of Isocitrate Dehydrogenase Mutation Status in Diffuse Glioma

Isocitrate dehydrogenase (IDH) mutation status is an important biomarker in the glioma-defining subtype and corresponding prognosis. This study proposes a straightforward method for 2-hydroxyglutarate (2-HG) quantification by MR spectroscopy for IDH mutation status detection and directly compares in...

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Autores principales: van Dijken, Bart R. J., Jeltema, Hanne-Rinck, Kłos, Justyna, van Laar, Peter Jan, Enting, Roelien H., Maatman, Ronald G. H. J., Bijsterveld, Klaas, Den Dunnen, Wilfred F. A., Dierckx, Rudi A., Sijens, Paul E., van der Hoorn, Anouk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487112/
https://www.ncbi.nlm.nih.gov/pubmed/37685329
http://dx.doi.org/10.3390/diagnostics13172791
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author van Dijken, Bart R. J.
Jeltema, Hanne-Rinck
Kłos, Justyna
van Laar, Peter Jan
Enting, Roelien H.
Maatman, Ronald G. H. J.
Bijsterveld, Klaas
Den Dunnen, Wilfred F. A.
Dierckx, Rudi A.
Sijens, Paul E.
van der Hoorn, Anouk
author_facet van Dijken, Bart R. J.
Jeltema, Hanne-Rinck
Kłos, Justyna
van Laar, Peter Jan
Enting, Roelien H.
Maatman, Ronald G. H. J.
Bijsterveld, Klaas
Den Dunnen, Wilfred F. A.
Dierckx, Rudi A.
Sijens, Paul E.
van der Hoorn, Anouk
author_sort van Dijken, Bart R. J.
collection PubMed
description Isocitrate dehydrogenase (IDH) mutation status is an important biomarker in the glioma-defining subtype and corresponding prognosis. This study proposes a straightforward method for 2-hydroxyglutarate (2-HG) quantification by MR spectroscopy for IDH mutation status detection and directly compares in vivo 2-HG MR spectroscopy with ex vivo 2-HG concentration measured in resected tumor tissue. Eleven patients with suspected lower-grade glioma (ten IDH1; one IDHwt) were prospectively included. Preoperatively, 3T point-resolved spectroscopy (PRESS) was acquired; 2-HG was measured as the percentage elevation of Glx3 (the sum of 2-HG and Glx) compared to Glx4. IDH mutation status was assessed by immunochemistry or direct sequencing. The ex vivo 2-HG concentration was determined in surgically obtained tissue specimens using gas chromatography–mass spectrometry. Pearson correlation was used for assessing the correlation between in vivo MR spectroscopy and ex vivo 2-HG concentration. MR spectroscopy was positive for 2-HG in eight patients, all of whom had IDH1 tumors. A strong correlation (r = 0.80, p = 0.003) between 2-HG MR spectroscopy and the ex vivo 2-HG concentration was found. This study shows in vivo 2-HG MR spectroscopy can non-invasively determine IDH status in glioma and demonstrates a strong correlation with ex vivo 2-HG concentration in patients with lower-grade glioma.
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spelling pubmed-104871122023-09-09 The Correlation of In Vivo MR Spectroscopy and Ex Vivo 2-Hydroxyglutarate Concentration for the Prediction of Isocitrate Dehydrogenase Mutation Status in Diffuse Glioma van Dijken, Bart R. J. Jeltema, Hanne-Rinck Kłos, Justyna van Laar, Peter Jan Enting, Roelien H. Maatman, Ronald G. H. J. Bijsterveld, Klaas Den Dunnen, Wilfred F. A. Dierckx, Rudi A. Sijens, Paul E. van der Hoorn, Anouk Diagnostics (Basel) Article Isocitrate dehydrogenase (IDH) mutation status is an important biomarker in the glioma-defining subtype and corresponding prognosis. This study proposes a straightforward method for 2-hydroxyglutarate (2-HG) quantification by MR spectroscopy for IDH mutation status detection and directly compares in vivo 2-HG MR spectroscopy with ex vivo 2-HG concentration measured in resected tumor tissue. Eleven patients with suspected lower-grade glioma (ten IDH1; one IDHwt) were prospectively included. Preoperatively, 3T point-resolved spectroscopy (PRESS) was acquired; 2-HG was measured as the percentage elevation of Glx3 (the sum of 2-HG and Glx) compared to Glx4. IDH mutation status was assessed by immunochemistry or direct sequencing. The ex vivo 2-HG concentration was determined in surgically obtained tissue specimens using gas chromatography–mass spectrometry. Pearson correlation was used for assessing the correlation between in vivo MR spectroscopy and ex vivo 2-HG concentration. MR spectroscopy was positive for 2-HG in eight patients, all of whom had IDH1 tumors. A strong correlation (r = 0.80, p = 0.003) between 2-HG MR spectroscopy and the ex vivo 2-HG concentration was found. This study shows in vivo 2-HG MR spectroscopy can non-invasively determine IDH status in glioma and demonstrates a strong correlation with ex vivo 2-HG concentration in patients with lower-grade glioma. MDPI 2023-08-29 /pmc/articles/PMC10487112/ /pubmed/37685329 http://dx.doi.org/10.3390/diagnostics13172791 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
van Dijken, Bart R. J.
Jeltema, Hanne-Rinck
Kłos, Justyna
van Laar, Peter Jan
Enting, Roelien H.
Maatman, Ronald G. H. J.
Bijsterveld, Klaas
Den Dunnen, Wilfred F. A.
Dierckx, Rudi A.
Sijens, Paul E.
van der Hoorn, Anouk
The Correlation of In Vivo MR Spectroscopy and Ex Vivo 2-Hydroxyglutarate Concentration for the Prediction of Isocitrate Dehydrogenase Mutation Status in Diffuse Glioma
title The Correlation of In Vivo MR Spectroscopy and Ex Vivo 2-Hydroxyglutarate Concentration for the Prediction of Isocitrate Dehydrogenase Mutation Status in Diffuse Glioma
title_full The Correlation of In Vivo MR Spectroscopy and Ex Vivo 2-Hydroxyglutarate Concentration for the Prediction of Isocitrate Dehydrogenase Mutation Status in Diffuse Glioma
title_fullStr The Correlation of In Vivo MR Spectroscopy and Ex Vivo 2-Hydroxyglutarate Concentration for the Prediction of Isocitrate Dehydrogenase Mutation Status in Diffuse Glioma
title_full_unstemmed The Correlation of In Vivo MR Spectroscopy and Ex Vivo 2-Hydroxyglutarate Concentration for the Prediction of Isocitrate Dehydrogenase Mutation Status in Diffuse Glioma
title_short The Correlation of In Vivo MR Spectroscopy and Ex Vivo 2-Hydroxyglutarate Concentration for the Prediction of Isocitrate Dehydrogenase Mutation Status in Diffuse Glioma
title_sort correlation of in vivo mr spectroscopy and ex vivo 2-hydroxyglutarate concentration for the prediction of isocitrate dehydrogenase mutation status in diffuse glioma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487112/
https://www.ncbi.nlm.nih.gov/pubmed/37685329
http://dx.doi.org/10.3390/diagnostics13172791
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