Cargando…
Beneficial Effects of Dietary Flaxseed Oil through Inflammation Pathways and Gut Microbiota in Streptozotocin-Induced Diabetic Mice
Flaxseed oil (FO) has displayed potential anti-diabetes properties by providing a high content of α-linolenic acid. However, the effects and mechanisms of FO on type 1 diabetes are still unclear. The present study aims to explore the effects of different doses of FO feeding on hepatic inflammation a...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487211/ https://www.ncbi.nlm.nih.gov/pubmed/37685162 http://dx.doi.org/10.3390/foods12173229 |
_version_ | 1785103185853022208 |
---|---|
author | Xia, Hui Wang, Ying Shi, Xiangling Liao, Wang Wang, Shaokang Sui, Jing Sun, Guiju |
author_facet | Xia, Hui Wang, Ying Shi, Xiangling Liao, Wang Wang, Shaokang Sui, Jing Sun, Guiju |
author_sort | Xia, Hui |
collection | PubMed |
description | Flaxseed oil (FO) has displayed potential anti-diabetes properties by providing a high content of α-linolenic acid. However, the effects and mechanisms of FO on type 1 diabetes are still unclear. The present study aims to explore the effects of different doses of FO feeding on hepatic inflammation and gut microbiota in streptozotocin-induced diabetic mice. Forty-eight six-week-old C57BL/6J male mice were divided into a control group (CON), a diabetic group (MOD), a diabetes with 7.0% w/w FO feeding group (FO-L), and a diabetes with 10.5% w/w FO feeding group (FO-H) for six weeks. The 7.0% w/w and 10.5% w/w FO feeding groups exhibited potential recovery of the number and size of pancreas tissues. The fasting blood glucose level was significantly decreased only after 4 weeks of feeding with 10.5% w/w FO in diabetic mice. The 10.5% w/w FO feeding group significantly decreased the postprandial blood glucose level of mice in the OGTT test. Hepatic glycogen levels were dramatically upregulated in the mice fed with both 7.0% w/w and 10.5% w/w FO. FO feeding significantly attenuated hepatic LPS, TNF-α, and IL-1β levels. In addition, we observed that 7.0% w/w and 10.5% w/w FO feedings notably downregulated hepatic gene and protein expressions of TLR4, MyD88, and P65. Furthermore, only 10.5% FO regulated fecal microbiota by increasing the relative abundance of the Bacteroidetes phylum, Lactococcus family, and Muribaculaceae and Streptococcaceae family and genus in streptozotocin-induced diabetic mice. Therefore, we conclude that FO feeding plays a role in anti-inflammation via the regulation of hepatic LPS/TLR4/MyD88 pathways and gut microbiota. In addition, different doses of FO supplementation may exhibit varying mechanisms in streptozotocin-induced mice. |
format | Online Article Text |
id | pubmed-10487211 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104872112023-09-09 Beneficial Effects of Dietary Flaxseed Oil through Inflammation Pathways and Gut Microbiota in Streptozotocin-Induced Diabetic Mice Xia, Hui Wang, Ying Shi, Xiangling Liao, Wang Wang, Shaokang Sui, Jing Sun, Guiju Foods Article Flaxseed oil (FO) has displayed potential anti-diabetes properties by providing a high content of α-linolenic acid. However, the effects and mechanisms of FO on type 1 diabetes are still unclear. The present study aims to explore the effects of different doses of FO feeding on hepatic inflammation and gut microbiota in streptozotocin-induced diabetic mice. Forty-eight six-week-old C57BL/6J male mice were divided into a control group (CON), a diabetic group (MOD), a diabetes with 7.0% w/w FO feeding group (FO-L), and a diabetes with 10.5% w/w FO feeding group (FO-H) for six weeks. The 7.0% w/w and 10.5% w/w FO feeding groups exhibited potential recovery of the number and size of pancreas tissues. The fasting blood glucose level was significantly decreased only after 4 weeks of feeding with 10.5% w/w FO in diabetic mice. The 10.5% w/w FO feeding group significantly decreased the postprandial blood glucose level of mice in the OGTT test. Hepatic glycogen levels were dramatically upregulated in the mice fed with both 7.0% w/w and 10.5% w/w FO. FO feeding significantly attenuated hepatic LPS, TNF-α, and IL-1β levels. In addition, we observed that 7.0% w/w and 10.5% w/w FO feedings notably downregulated hepatic gene and protein expressions of TLR4, MyD88, and P65. Furthermore, only 10.5% FO regulated fecal microbiota by increasing the relative abundance of the Bacteroidetes phylum, Lactococcus family, and Muribaculaceae and Streptococcaceae family and genus in streptozotocin-induced diabetic mice. Therefore, we conclude that FO feeding plays a role in anti-inflammation via the regulation of hepatic LPS/TLR4/MyD88 pathways and gut microbiota. In addition, different doses of FO supplementation may exhibit varying mechanisms in streptozotocin-induced mice. MDPI 2023-08-28 /pmc/articles/PMC10487211/ /pubmed/37685162 http://dx.doi.org/10.3390/foods12173229 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xia, Hui Wang, Ying Shi, Xiangling Liao, Wang Wang, Shaokang Sui, Jing Sun, Guiju Beneficial Effects of Dietary Flaxseed Oil through Inflammation Pathways and Gut Microbiota in Streptozotocin-Induced Diabetic Mice |
title | Beneficial Effects of Dietary Flaxseed Oil through Inflammation Pathways and Gut Microbiota in Streptozotocin-Induced Diabetic Mice |
title_full | Beneficial Effects of Dietary Flaxseed Oil through Inflammation Pathways and Gut Microbiota in Streptozotocin-Induced Diabetic Mice |
title_fullStr | Beneficial Effects of Dietary Flaxseed Oil through Inflammation Pathways and Gut Microbiota in Streptozotocin-Induced Diabetic Mice |
title_full_unstemmed | Beneficial Effects of Dietary Flaxseed Oil through Inflammation Pathways and Gut Microbiota in Streptozotocin-Induced Diabetic Mice |
title_short | Beneficial Effects of Dietary Flaxseed Oil through Inflammation Pathways and Gut Microbiota in Streptozotocin-Induced Diabetic Mice |
title_sort | beneficial effects of dietary flaxseed oil through inflammation pathways and gut microbiota in streptozotocin-induced diabetic mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487211/ https://www.ncbi.nlm.nih.gov/pubmed/37685162 http://dx.doi.org/10.3390/foods12173229 |
work_keys_str_mv | AT xiahui beneficialeffectsofdietaryflaxseedoilthroughinflammationpathwaysandgutmicrobiotainstreptozotocininduceddiabeticmice AT wangying beneficialeffectsofdietaryflaxseedoilthroughinflammationpathwaysandgutmicrobiotainstreptozotocininduceddiabeticmice AT shixiangling beneficialeffectsofdietaryflaxseedoilthroughinflammationpathwaysandgutmicrobiotainstreptozotocininduceddiabeticmice AT liaowang beneficialeffectsofdietaryflaxseedoilthroughinflammationpathwaysandgutmicrobiotainstreptozotocininduceddiabeticmice AT wangshaokang beneficialeffectsofdietaryflaxseedoilthroughinflammationpathwaysandgutmicrobiotainstreptozotocininduceddiabeticmice AT suijing beneficialeffectsofdietaryflaxseedoilthroughinflammationpathwaysandgutmicrobiotainstreptozotocininduceddiabeticmice AT sunguiju beneficialeffectsofdietaryflaxseedoilthroughinflammationpathwaysandgutmicrobiotainstreptozotocininduceddiabeticmice |