Mechanisms used for cDNA synthesis and site-specific integration of RNA into DNA genomes by a reverse transcriptase-Cas1 fusion protein

Reverse transcriptase-Cas1 (RT-Cas1) fusion proteins found in some CRISPR systems enable spacer acquisition from both RNA and DNA, but the mechanism of RNA spacer acquisition has remained unclear. Here, we found Marinomonas mediterranea RT-Cas1/Cas2 adds short 3’-DNA (dN) tails to RNA protospacers e...

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Detalles Bibliográficos
Autores principales: Mohr, Georg, Yao, Jun, Park, Seung Kuk, Markham, Laura M., Lambowitz, Alan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10491204/
https://www.ncbi.nlm.nih.gov/pubmed/37693417
http://dx.doi.org/10.1101/2023.09.01.555893

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10491204/
https://www.ncbi.nlm.nih.gov/pubmed/37693417
http://dx.doi.org/10.1101/2023.09.01.555893

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