Protocol to study projection-specific circuits in the basal ganglia of adult mice using viral vector tracing, optogenetics, and patch-clamp technique

Analysis of synaptic strength and plasticity provides functional insights of complicated neural circuits. Here, we describe steps for cell- and projection-specific optogenetic manipulation of divergent basal ganglia circuits using anterograde and retrograde viral vectors. We quantitatively analyze s...

Descripción completa

Detalles Bibliográficos
Autores principales: Ji, Ya-Wei, Xu, Xiang-Ying, Yin, Cui, Zhou, Chunyi, Xiao, Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10491855/
https://www.ncbi.nlm.nih.gov/pubmed/37660296
http://dx.doi.org/10.1016/j.xpro.2023.102551
_version_ 1785104149066547200
author Ji, Ya-Wei
Xu, Xiang-Ying
Yin, Cui
Zhou, Chunyi
Xiao, Cheng
author_facet Ji, Ya-Wei
Xu, Xiang-Ying
Yin, Cui
Zhou, Chunyi
Xiao, Cheng
author_sort Ji, Ya-Wei
collection PubMed
description Analysis of synaptic strength and plasticity provides functional insights of complicated neural circuits. Here, we describe steps for cell- and projection-specific optogenetic manipulation of divergent basal ganglia circuits using anterograde and retrograde viral vectors. We quantitatively analyze synaptic function of these circuits utilizing a patch-clamp technique. This protocol is applicable to probe potential circuit targets for treatment of brain diseases. For complete details on the use and execution of this protocol, please refer to Ji et al.(1)
format Online
Article
Text
id pubmed-10491855
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-104918552023-09-10 Protocol to study projection-specific circuits in the basal ganglia of adult mice using viral vector tracing, optogenetics, and patch-clamp technique Ji, Ya-Wei Xu, Xiang-Ying Yin, Cui Zhou, Chunyi Xiao, Cheng STAR Protoc Protocol Analysis of synaptic strength and plasticity provides functional insights of complicated neural circuits. Here, we describe steps for cell- and projection-specific optogenetic manipulation of divergent basal ganglia circuits using anterograde and retrograde viral vectors. We quantitatively analyze synaptic function of these circuits utilizing a patch-clamp technique. This protocol is applicable to probe potential circuit targets for treatment of brain diseases. For complete details on the use and execution of this protocol, please refer to Ji et al.(1) Elsevier 2023-09-02 /pmc/articles/PMC10491855/ /pubmed/37660296 http://dx.doi.org/10.1016/j.xpro.2023.102551 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ji, Ya-Wei
Xu, Xiang-Ying
Yin, Cui
Zhou, Chunyi
Xiao, Cheng
Protocol to study projection-specific circuits in the basal ganglia of adult mice using viral vector tracing, optogenetics, and patch-clamp technique
title Protocol to study projection-specific circuits in the basal ganglia of adult mice using viral vector tracing, optogenetics, and patch-clamp technique
title_full Protocol to study projection-specific circuits in the basal ganglia of adult mice using viral vector tracing, optogenetics, and patch-clamp technique
title_fullStr Protocol to study projection-specific circuits in the basal ganglia of adult mice using viral vector tracing, optogenetics, and patch-clamp technique
title_full_unstemmed Protocol to study projection-specific circuits in the basal ganglia of adult mice using viral vector tracing, optogenetics, and patch-clamp technique
title_short Protocol to study projection-specific circuits in the basal ganglia of adult mice using viral vector tracing, optogenetics, and patch-clamp technique
title_sort protocol to study projection-specific circuits in the basal ganglia of adult mice using viral vector tracing, optogenetics, and patch-clamp technique
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10491855/
https://www.ncbi.nlm.nih.gov/pubmed/37660296
http://dx.doi.org/10.1016/j.xpro.2023.102551
work_keys_str_mv AT jiyawei protocoltostudyprojectionspecificcircuitsinthebasalgangliaofadultmiceusingviralvectortracingoptogeneticsandpatchclamptechnique
AT xuxiangying protocoltostudyprojectionspecificcircuitsinthebasalgangliaofadultmiceusingviralvectortracingoptogeneticsandpatchclamptechnique
AT yincui protocoltostudyprojectionspecificcircuitsinthebasalgangliaofadultmiceusingviralvectortracingoptogeneticsandpatchclamptechnique
AT zhouchunyi protocoltostudyprojectionspecificcircuitsinthebasalgangliaofadultmiceusingviralvectortracingoptogeneticsandpatchclamptechnique
AT xiaocheng protocoltostudyprojectionspecificcircuitsinthebasalgangliaofadultmiceusingviralvectortracingoptogeneticsandpatchclamptechnique

Ejemplares similares