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Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasma
A fast, simple, and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established for the quantification of safinamide in rat plasma. Plasma samples were treated with acetonitrile for protein precipitation, and diazepam was used as an internal standar...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493267/ https://www.ncbi.nlm.nih.gov/pubmed/37701033 http://dx.doi.org/10.3389/fphar.2023.1211383 |
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author | Wang, Ying Zhao, Guan-An Li, Xia Zhang, En Tan, Wei Chen, Jia-qi |
author_facet | Wang, Ying Zhao, Guan-An Li, Xia Zhang, En Tan, Wei Chen, Jia-qi |
author_sort | Wang, Ying |
collection | PubMed |
description | A fast, simple, and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established for the quantification of safinamide in rat plasma. Plasma samples were treated with acetonitrile for protein precipitation, and diazepam was used as an internal standard (IS). The analytes were separated on an Acquity UPLC C18 (2.1 mm × 50 mm, 1.7 μm) chromatographic column with gradient elution using a mobile phase (0.1% formic acid-acetonitrile). Then, the eluates were detected by electrospray ionization (ESI) in positive ion mode. The analytes were quantified by multiple reaction monitoring (MRM) using the transition m/z 303.3→215.0 of safinamide and m/z 285.0→154.0 of IS. Safinamide had good linearity in the concentration range of 1.0–2000 ng/mL, and the lower limit of quantitation (LLOQ) was 1.0 ng/mL. The intra- and inter-day precision and accuracy of safinamide were less than 7.63%, while the average recovery rate was 92.98%–100.29%. The method was validated to be stable and had low noise, short chromatographic run time, wide linear range, small sample volumes, low sample injection volumes, and high sensitivity. Therefore, it can be used in pharmacokinetics and preclinical and clinical studies. |
format | Online Article Text |
id | pubmed-10493267 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104932672023-09-12 Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasma Wang, Ying Zhao, Guan-An Li, Xia Zhang, En Tan, Wei Chen, Jia-qi Front Pharmacol Pharmacology A fast, simple, and sensitive ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established for the quantification of safinamide in rat plasma. Plasma samples were treated with acetonitrile for protein precipitation, and diazepam was used as an internal standard (IS). The analytes were separated on an Acquity UPLC C18 (2.1 mm × 50 mm, 1.7 μm) chromatographic column with gradient elution using a mobile phase (0.1% formic acid-acetonitrile). Then, the eluates were detected by electrospray ionization (ESI) in positive ion mode. The analytes were quantified by multiple reaction monitoring (MRM) using the transition m/z 303.3→215.0 of safinamide and m/z 285.0→154.0 of IS. Safinamide had good linearity in the concentration range of 1.0–2000 ng/mL, and the lower limit of quantitation (LLOQ) was 1.0 ng/mL. The intra- and inter-day precision and accuracy of safinamide were less than 7.63%, while the average recovery rate was 92.98%–100.29%. The method was validated to be stable and had low noise, short chromatographic run time, wide linear range, small sample volumes, low sample injection volumes, and high sensitivity. Therefore, it can be used in pharmacokinetics and preclinical and clinical studies. Frontiers Media S.A. 2023-08-28 /pmc/articles/PMC10493267/ /pubmed/37701033 http://dx.doi.org/10.3389/fphar.2023.1211383 Text en Copyright © 2023 Wang, Zhao, Li, Zhang, Tan and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Wang, Ying Zhao, Guan-An Li, Xia Zhang, En Tan, Wei Chen, Jia-qi Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasma |
title | Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasma |
title_full | Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasma |
title_fullStr | Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasma |
title_full_unstemmed | Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasma |
title_short | Establishment of a sensitive UPLC-MS/MS method to quantify safinamide in rat plasma |
title_sort | establishment of a sensitive uplc-ms/ms method to quantify safinamide in rat plasma |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493267/ https://www.ncbi.nlm.nih.gov/pubmed/37701033 http://dx.doi.org/10.3389/fphar.2023.1211383 |
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