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Protocol for in vivo and in vitro activation of NLRP3 inflammasome in mice using monosodium urate

Although intraperitoneal injection of monosodium urate (MSU) is an effective model for studying peritonitis, its establishment remains challenging. Here, we present a protocol for using MSU to activate the NLRP3 inflammasome in bone-marrow-derived macrophages (BMDMs) and to induce peritonitis in mic...

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Detalles Bibliográficos
Autores principales: Liu, Didi, Huang, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493585/
https://www.ncbi.nlm.nih.gov/pubmed/37682717
http://dx.doi.org/10.1016/j.xpro.2023.102554