Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila

Exploring early embryonic gene expression is challenging due to the rate of development and the limited material available. Here, we present a protocol for ordering Drosophila embryos along a developmental pseudo-time trajectory and determining the sex of the embryos using RNA-seq data. We describe...

Descripción completa

Detalles Bibliográficos
Autores principales: Pérez-Mojica, J. Eduardo, Enders, Lennart, Lau, Kin H., Lempradl, Adelheid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493594/
https://www.ncbi.nlm.nih.gov/pubmed/37682716
http://dx.doi.org/10.1016/j.xpro.2023.102535

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493594/
https://www.ncbi.nlm.nih.gov/pubmed/37682716
http://dx.doi.org/10.1016/j.xpro.2023.102535

Ejemplares similares