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Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
Exploring early embryonic gene expression is challenging due to the rate of development and the limited material available. Here, we present a protocol for ordering Drosophila embryos along a developmental pseudo-time trajectory and determining the sex of the embryos using RNA-seq data. We describe...
Autores principales: | Pérez-Mojica, J. Eduardo, Enders, Lennart, Lau, Kin H., Lempradl, Adelheid |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493594/ https://www.ncbi.nlm.nih.gov/pubmed/37682716 http://dx.doi.org/10.1016/j.xpro.2023.102535 |
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