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The neurotrophic activities of brain‐derived neurotrophic factor are potentiated by binding with apigenin, a common flavone in vegetables, in stimulating the receptor signaling
AIMS: We aimed to identify the neurotrophic activities of apigenin (4′,5,7‐trihydroxyflavone) via its coordination with brain‐derived neurotrophic factor (BNDF) and an elevated signaling of tyrosine kinase receptor B (Trk B receptor). METHODS: The direct binding of apigenin to BDNF was validated by...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493664/ https://www.ncbi.nlm.nih.gov/pubmed/37101380 http://dx.doi.org/10.1111/cns.14230 |
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author | Gao, Alex Xiong Xia, Tracy Chen‐Xi Lin, Lish Sheng‐Ying Dong, Tina Ting‐Xia Tsim, Karl Wah‐Keung |
author_facet | Gao, Alex Xiong Xia, Tracy Chen‐Xi Lin, Lish Sheng‐Ying Dong, Tina Ting‐Xia Tsim, Karl Wah‐Keung |
author_sort | Gao, Alex Xiong |
collection | PubMed |
description | AIMS: We aimed to identify the neurotrophic activities of apigenin (4′,5,7‐trihydroxyflavone) via its coordination with brain‐derived neurotrophic factor (BNDF) and an elevated signaling of tyrosine kinase receptor B (Trk B receptor). METHODS: The direct binding of apigenin to BDNF was validated by ultrafiltration and biacore assay. Neurogenesis, triggered by apigenin and/or BDNF, was determined in cultured SH‐SY5Y cells and rat cortical neurons. The amyloid‐beta (Aβ)(25‐35)‐induced cellular stress was revealed by propidium iodide staining, mitochondrial membrane potential, bioenergetic analysis, and formation of reactive oxygen species levels. Activation of Trk B signaling was tested by western blotting. RESULTS: Apigenin and BDNF synergistically maintained the cell viability and promoted neurite outgrowth of cultured neurons. In addition, the BDNF‐induced neurogenesis of cultured neurons was markedly potentiated by applied apigenin, including the induced expressions of neurofilaments, PSD‐95 and synaptotagmin. Moreover, the synergy of apigenin and BDNF alleviated the (Aβ)(25‐35)‐induced cytotoxicity and mitochondrial dysfunction. The synergy could be accounted by phosphorylation of Trk B receptor, and which was fully blocked by a Trk inhibitor K252a. CONCLUSION: Apigenin potentiates the neurotrophic activities of BDNF through direct binding, which may serve as a possible treatment for its curative efficiency in neurodegenerative diseases and depression. |
format | Online Article Text |
id | pubmed-10493664 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104936642023-09-12 The neurotrophic activities of brain‐derived neurotrophic factor are potentiated by binding with apigenin, a common flavone in vegetables, in stimulating the receptor signaling Gao, Alex Xiong Xia, Tracy Chen‐Xi Lin, Lish Sheng‐Ying Dong, Tina Ting‐Xia Tsim, Karl Wah‐Keung CNS Neurosci Ther Original Articles AIMS: We aimed to identify the neurotrophic activities of apigenin (4′,5,7‐trihydroxyflavone) via its coordination with brain‐derived neurotrophic factor (BNDF) and an elevated signaling of tyrosine kinase receptor B (Trk B receptor). METHODS: The direct binding of apigenin to BDNF was validated by ultrafiltration and biacore assay. Neurogenesis, triggered by apigenin and/or BDNF, was determined in cultured SH‐SY5Y cells and rat cortical neurons. The amyloid‐beta (Aβ)(25‐35)‐induced cellular stress was revealed by propidium iodide staining, mitochondrial membrane potential, bioenergetic analysis, and formation of reactive oxygen species levels. Activation of Trk B signaling was tested by western blotting. RESULTS: Apigenin and BDNF synergistically maintained the cell viability and promoted neurite outgrowth of cultured neurons. In addition, the BDNF‐induced neurogenesis of cultured neurons was markedly potentiated by applied apigenin, including the induced expressions of neurofilaments, PSD‐95 and synaptotagmin. Moreover, the synergy of apigenin and BDNF alleviated the (Aβ)(25‐35)‐induced cytotoxicity and mitochondrial dysfunction. The synergy could be accounted by phosphorylation of Trk B receptor, and which was fully blocked by a Trk inhibitor K252a. CONCLUSION: Apigenin potentiates the neurotrophic activities of BDNF through direct binding, which may serve as a possible treatment for its curative efficiency in neurodegenerative diseases and depression. John Wiley and Sons Inc. 2023-04-26 /pmc/articles/PMC10493664/ /pubmed/37101380 http://dx.doi.org/10.1111/cns.14230 Text en © 2023 The Authors. CNS Neuroscience & Therapeutics published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Gao, Alex Xiong Xia, Tracy Chen‐Xi Lin, Lish Sheng‐Ying Dong, Tina Ting‐Xia Tsim, Karl Wah‐Keung The neurotrophic activities of brain‐derived neurotrophic factor are potentiated by binding with apigenin, a common flavone in vegetables, in stimulating the receptor signaling |
title | The neurotrophic activities of brain‐derived neurotrophic factor are potentiated by binding with apigenin, a common flavone in vegetables, in stimulating the receptor signaling |
title_full | The neurotrophic activities of brain‐derived neurotrophic factor are potentiated by binding with apigenin, a common flavone in vegetables, in stimulating the receptor signaling |
title_fullStr | The neurotrophic activities of brain‐derived neurotrophic factor are potentiated by binding with apigenin, a common flavone in vegetables, in stimulating the receptor signaling |
title_full_unstemmed | The neurotrophic activities of brain‐derived neurotrophic factor are potentiated by binding with apigenin, a common flavone in vegetables, in stimulating the receptor signaling |
title_short | The neurotrophic activities of brain‐derived neurotrophic factor are potentiated by binding with apigenin, a common flavone in vegetables, in stimulating the receptor signaling |
title_sort | neurotrophic activities of brain‐derived neurotrophic factor are potentiated by binding with apigenin, a common flavone in vegetables, in stimulating the receptor signaling |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493664/ https://www.ncbi.nlm.nih.gov/pubmed/37101380 http://dx.doi.org/10.1111/cns.14230 |
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