tgCRISPRi: efficient gene knock-down using truncated gRNAs and catalytically active Cas9

CRISPR-interference (CRISPRi), a highly effective method for silencing genes in mammalian cells, employs an enzymatically dead form of Cas9 (dCas9) complexed with one or more guide RNAs (gRNAs) with 20 nucleotides (nt) of complementarity to transcription initiation sites of target genes. Such gRNA/d...

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Detalles Bibliográficos
Autores principales: Auradkar, Ankush, Guichard, Annabel, Kaduwal, Saluja, Sneider, Marketta, Bier, Ethan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495392/
https://www.ncbi.nlm.nih.gov/pubmed/37696787
http://dx.doi.org/10.1038/s41467-023-40836-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495392/
https://www.ncbi.nlm.nih.gov/pubmed/37696787
http://dx.doi.org/10.1038/s41467-023-40836-3

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