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Metabolic and transcriptomic profiling during wheat seed development under progressive drought conditions

Globally, bread wheat (Triticum aestivum) is one of the most important staple foods; when exposed to drought, wheat yields decline. Although much research has been performed to generate higher yield wheat cultivars, there have been few studies on improving end-product quality under drought stress, e...

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Autores principales: Mega, Ryosuke, Kim, June-Sik, Tanaka, Hiroyuki, Ishii, Takayoshi, Abe, Fumitaka, Okamoto, Masanori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495411/
https://www.ncbi.nlm.nih.gov/pubmed/37696863
http://dx.doi.org/10.1038/s41598-023-42093-2
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author Mega, Ryosuke
Kim, June-Sik
Tanaka, Hiroyuki
Ishii, Takayoshi
Abe, Fumitaka
Okamoto, Masanori
author_facet Mega, Ryosuke
Kim, June-Sik
Tanaka, Hiroyuki
Ishii, Takayoshi
Abe, Fumitaka
Okamoto, Masanori
author_sort Mega, Ryosuke
collection PubMed
description Globally, bread wheat (Triticum aestivum) is one of the most important staple foods; when exposed to drought, wheat yields decline. Although much research has been performed to generate higher yield wheat cultivars, there have been few studies on improving end-product quality under drought stress, even though wheat is processed into flour to produce so many foods, such as bread, noodles, pancakes, cakes, and cookies. Recently, wheat cultivation has been affected by severe drought caused by global climate change. In previous studies, seed shrinkage was observed in wheat exposed to continuous drought stress during seed development. In this study, we investigated how progressive drought stress affected seed development by metabolomic and transcriptomic analyses. Metabolite profiling revealed the drought-sensitive line reduced accumulation of proline and sugar compared with the water-saving, drought-tolerant transgenic line overexpressing the abscisic acid receptor TaPYL4 under drought conditions in spikelets with developing seeds. Meanwhile, the expressions of genes involved in translation, starch biosynthesis, and proline and arginine biosynthesis was downregulated in the drought-sensitive line. These findings suggest that seed shrinkage, exemplifying a deficiency in endosperm, arose from the hindered biosynthesis of crucial components including seed storage proteins, starch, amino acids, and sugars, ultimately leading to their inadequate accumulation within spikelets. Water-saving drought tolerant traits of wheat would aid in supporting seed formation under drought conditions.
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spelling pubmed-104954112023-09-13 Metabolic and transcriptomic profiling during wheat seed development under progressive drought conditions Mega, Ryosuke Kim, June-Sik Tanaka, Hiroyuki Ishii, Takayoshi Abe, Fumitaka Okamoto, Masanori Sci Rep Article Globally, bread wheat (Triticum aestivum) is one of the most important staple foods; when exposed to drought, wheat yields decline. Although much research has been performed to generate higher yield wheat cultivars, there have been few studies on improving end-product quality under drought stress, even though wheat is processed into flour to produce so many foods, such as bread, noodles, pancakes, cakes, and cookies. Recently, wheat cultivation has been affected by severe drought caused by global climate change. In previous studies, seed shrinkage was observed in wheat exposed to continuous drought stress during seed development. In this study, we investigated how progressive drought stress affected seed development by metabolomic and transcriptomic analyses. Metabolite profiling revealed the drought-sensitive line reduced accumulation of proline and sugar compared with the water-saving, drought-tolerant transgenic line overexpressing the abscisic acid receptor TaPYL4 under drought conditions in spikelets with developing seeds. Meanwhile, the expressions of genes involved in translation, starch biosynthesis, and proline and arginine biosynthesis was downregulated in the drought-sensitive line. These findings suggest that seed shrinkage, exemplifying a deficiency in endosperm, arose from the hindered biosynthesis of crucial components including seed storage proteins, starch, amino acids, and sugars, ultimately leading to their inadequate accumulation within spikelets. Water-saving drought tolerant traits of wheat would aid in supporting seed formation under drought conditions. Nature Publishing Group UK 2023-09-11 /pmc/articles/PMC10495411/ /pubmed/37696863 http://dx.doi.org/10.1038/s41598-023-42093-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Mega, Ryosuke
Kim, June-Sik
Tanaka, Hiroyuki
Ishii, Takayoshi
Abe, Fumitaka
Okamoto, Masanori
Metabolic and transcriptomic profiling during wheat seed development under progressive drought conditions
title Metabolic and transcriptomic profiling during wheat seed development under progressive drought conditions
title_full Metabolic and transcriptomic profiling during wheat seed development under progressive drought conditions
title_fullStr Metabolic and transcriptomic profiling during wheat seed development under progressive drought conditions
title_full_unstemmed Metabolic and transcriptomic profiling during wheat seed development under progressive drought conditions
title_short Metabolic and transcriptomic profiling during wheat seed development under progressive drought conditions
title_sort metabolic and transcriptomic profiling during wheat seed development under progressive drought conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495411/
https://www.ncbi.nlm.nih.gov/pubmed/37696863
http://dx.doi.org/10.1038/s41598-023-42093-2
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