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Novel T cell/organoid culture system allows ex vivo modeling of intestinal graft-versus-host disease

Acute graft-versus-host disease (GvHD) remains the biggest clinical challenge and prognosis-determining complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Donor T cells are acceptedly key mediators of alloreactivity against host tissues and here especially the gut. In...

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Autores principales: Matthe, Diana M., Dinkel, Martin, Schmid, Benjamin, Vogler, Tina, Neurath, Markus F., Poeck, Hendrik, Neufert, Clemens, Büttner-Herold, Maike, Hildner, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495981/
https://www.ncbi.nlm.nih.gov/pubmed/37705975
http://dx.doi.org/10.3389/fimmu.2023.1253514
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author Matthe, Diana M.
Dinkel, Martin
Schmid, Benjamin
Vogler, Tina
Neurath, Markus F.
Poeck, Hendrik
Neufert, Clemens
Büttner-Herold, Maike
Hildner, Kai
author_facet Matthe, Diana M.
Dinkel, Martin
Schmid, Benjamin
Vogler, Tina
Neurath, Markus F.
Poeck, Hendrik
Neufert, Clemens
Büttner-Herold, Maike
Hildner, Kai
author_sort Matthe, Diana M.
collection PubMed
description Acute graft-versus-host disease (GvHD) remains the biggest clinical challenge and prognosis-determining complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Donor T cells are acceptedly key mediators of alloreactivity against host tissues and here especially the gut. In support of previous studies, we found that the intestinal intra-epithelial lymphocyte (IEL) compartment was dynamically regulated in the course of MHC class I full mismatch allo-HSCT. However, while intestinal epithelial cell (IEC) damage endangers the integrity of the intestinal barrier and is a core signature of intestinal GvHD, the question whether and to what degree IELs are contributing to IEC dysregulation is poorly understood. To study lymphoepithelial interaction, we employed a novel ex vivo T cell/organoid co-culture model system. Here, allogeneic intra-epithelial T cells were superior in inducing IEC death compared to syngeneic IEL and allogeneic non-IEL T cells. The ability to induce IEC death was predominately confined to TCRβ+ T cells and was executed in a largely IFNγ-dependent manner. Alloreactivity required a diverse T cell receptor (TCR) repertoire since IELs genetically modified to express a TCR restricted to a single, non-endogenous antigen failed to mediate IEC pathology. Interestingly, minor histocompatibility antigen (miHA) mismatch was sufficient to elicit IEL-driven IEC damage. Finally, advanced live cell imaging analyses uncovered that alloreactive IELs patrolled smaller areas within intestinal organoids compared to syngeneic controls, indicating their unique migratory properties within allogeneic IECs. Together, we provide here experimental evidence for the utility of a co-culture system to model the cellular and molecular characteristics of the crosstalk between IELs and IEC in an allogeneic setting ex vivo. In the light of the emerging concept of dysregulated immune-epithelial homeostasis as a core aspect of intestinal GvHD, this approach represents a novel experimental system to e.g. screen therapeutic strategies for their potential to normalize T cell/IEC- interaction. Hence, analyses in pre-clinical in vivo allo-HSCT model systems may be restricted to hereby positively selected, promising approaches.
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spelling pubmed-104959812023-09-13 Novel T cell/organoid culture system allows ex vivo modeling of intestinal graft-versus-host disease Matthe, Diana M. Dinkel, Martin Schmid, Benjamin Vogler, Tina Neurath, Markus F. Poeck, Hendrik Neufert, Clemens Büttner-Herold, Maike Hildner, Kai Front Immunol Immunology Acute graft-versus-host disease (GvHD) remains the biggest clinical challenge and prognosis-determining complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Donor T cells are acceptedly key mediators of alloreactivity against host tissues and here especially the gut. In support of previous studies, we found that the intestinal intra-epithelial lymphocyte (IEL) compartment was dynamically regulated in the course of MHC class I full mismatch allo-HSCT. However, while intestinal epithelial cell (IEC) damage endangers the integrity of the intestinal barrier and is a core signature of intestinal GvHD, the question whether and to what degree IELs are contributing to IEC dysregulation is poorly understood. To study lymphoepithelial interaction, we employed a novel ex vivo T cell/organoid co-culture model system. Here, allogeneic intra-epithelial T cells were superior in inducing IEC death compared to syngeneic IEL and allogeneic non-IEL T cells. The ability to induce IEC death was predominately confined to TCRβ+ T cells and was executed in a largely IFNγ-dependent manner. Alloreactivity required a diverse T cell receptor (TCR) repertoire since IELs genetically modified to express a TCR restricted to a single, non-endogenous antigen failed to mediate IEC pathology. Interestingly, minor histocompatibility antigen (miHA) mismatch was sufficient to elicit IEL-driven IEC damage. Finally, advanced live cell imaging analyses uncovered that alloreactive IELs patrolled smaller areas within intestinal organoids compared to syngeneic controls, indicating their unique migratory properties within allogeneic IECs. Together, we provide here experimental evidence for the utility of a co-culture system to model the cellular and molecular characteristics of the crosstalk between IELs and IEC in an allogeneic setting ex vivo. In the light of the emerging concept of dysregulated immune-epithelial homeostasis as a core aspect of intestinal GvHD, this approach represents a novel experimental system to e.g. screen therapeutic strategies for their potential to normalize T cell/IEC- interaction. Hence, analyses in pre-clinical in vivo allo-HSCT model systems may be restricted to hereby positively selected, promising approaches. Frontiers Media S.A. 2023-08-29 /pmc/articles/PMC10495981/ /pubmed/37705975 http://dx.doi.org/10.3389/fimmu.2023.1253514 Text en Copyright © 2023 Matthe, Dinkel, Schmid, Vogler, Neurath, Poeck, Neufert, Büttner-Herold and Hildner https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Matthe, Diana M.
Dinkel, Martin
Schmid, Benjamin
Vogler, Tina
Neurath, Markus F.
Poeck, Hendrik
Neufert, Clemens
Büttner-Herold, Maike
Hildner, Kai
Novel T cell/organoid culture system allows ex vivo modeling of intestinal graft-versus-host disease
title Novel T cell/organoid culture system allows ex vivo modeling of intestinal graft-versus-host disease
title_full Novel T cell/organoid culture system allows ex vivo modeling of intestinal graft-versus-host disease
title_fullStr Novel T cell/organoid culture system allows ex vivo modeling of intestinal graft-versus-host disease
title_full_unstemmed Novel T cell/organoid culture system allows ex vivo modeling of intestinal graft-versus-host disease
title_short Novel T cell/organoid culture system allows ex vivo modeling of intestinal graft-versus-host disease
title_sort novel t cell/organoid culture system allows ex vivo modeling of intestinal graft-versus-host disease
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495981/
https://www.ncbi.nlm.nih.gov/pubmed/37705975
http://dx.doi.org/10.3389/fimmu.2023.1253514
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