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The measurement of true initial rates is not always absolutely necessary to estimate enzyme kinetic parameters
In the chapters dealing with enzyme reactions, the authors of all Biochemistry textbooks and of even more specialized texts consider that the characteristic parameters (k(cat) and K(m)) must be determined under initial or steady-state rate conditions. This implies the transformation of a very limite...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497622/ https://www.ncbi.nlm.nih.gov/pubmed/37699921 http://dx.doi.org/10.1038/s41598-023-41805-y |