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Cell type–specific labeling of newly synthesized proteins by puromycin inactivation

Puromycin and its derivative O-propargyl puromycin (OPP) have recently found widespread use in detecting nascent proteins. Use of these metabolic labels in complex mixtures of cells leads to indiscriminate tagging of nascent proteomes independent of cell type. Here, we show how a widely used mammali...

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Detalles Bibliográficos
Autores principales: Cabrera-Cabrera, Florencia, Tull, Helena, Capuana, Roberta, Kasvandik, Sergo, Timmusk, Tõnis, Koppel, Indrek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497999/
https://www.ncbi.nlm.nih.gov/pubmed/37543363
http://dx.doi.org/10.1016/j.jbc.2023.105129