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Translation of biophysical environment in bone into dynamic cell culture under flow for bone tissue engineering

Bone is a dynamic environment where osteocytes, osteoblasts, and mesenchymal stem/progenitor cells perceive mechanical cues and regulate bone metabolism accordingly. In particular, interstitial fluid flow in bone and bone marrow serves as a primary biophysical stimulus, which regulates the growth an...

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Autores principales: Yamada, Shuntaro, Ockermann, Philipp Niklas, Schwarz, Thomas, Mustafa, Kamal, Hansmann, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10498129/
https://www.ncbi.nlm.nih.gov/pubmed/37711188
http://dx.doi.org/10.1016/j.csbj.2023.08.008
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author Yamada, Shuntaro
Ockermann, Philipp Niklas
Schwarz, Thomas
Mustafa, Kamal
Hansmann, Jan
author_facet Yamada, Shuntaro
Ockermann, Philipp Niklas
Schwarz, Thomas
Mustafa, Kamal
Hansmann, Jan
author_sort Yamada, Shuntaro
collection PubMed
description Bone is a dynamic environment where osteocytes, osteoblasts, and mesenchymal stem/progenitor cells perceive mechanical cues and regulate bone metabolism accordingly. In particular, interstitial fluid flow in bone and bone marrow serves as a primary biophysical stimulus, which regulates the growth and fate of the cellular components of bone. The processes of mechano-sensory and -transduction towards bone formation have been well studied mainly in vivo as well as in two-dimensional (2D) dynamic cell culture platforms, which elucidated mechanically induced osteogenesis starting with anabolic responses, such as production of nitrogen oxide and prostaglandins followed by the activation of canonical Wnt signaling, upon mechanosensation. The knowledge has been now translated into regenerative medicine, particularly into the field of bone tissue engineering, where multipotent stem cells are combined with three-dimensional (3D) scaffolding biomaterials to produce transplantable constructs for bone regeneration. In the presence of 3D scaffolds, the importance of suitable dynamic cell culture platforms increases further not only to improve mass transfer inside the scaffolds but to provide appropriate biophysical cues to guide cell fate. In principle, the concept of dynamic cell culture platforms is rooted to bone mechanobiology. Therefore, this review primarily focuses on biophysical environment in bone and its translation into dynamic cell culture platforms commonly used for 2D and 3D cell expansion, including their advancement, challenges, and future perspectives. Additionally, it provides the literature review of recent empirical studies using 2D and 3D flow-based dynamic cell culture systems for bone tissue engineering.
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spelling pubmed-104981292023-09-14 Translation of biophysical environment in bone into dynamic cell culture under flow for bone tissue engineering Yamada, Shuntaro Ockermann, Philipp Niklas Schwarz, Thomas Mustafa, Kamal Hansmann, Jan Comput Struct Biotechnol J Review Article Bone is a dynamic environment where osteocytes, osteoblasts, and mesenchymal stem/progenitor cells perceive mechanical cues and regulate bone metabolism accordingly. In particular, interstitial fluid flow in bone and bone marrow serves as a primary biophysical stimulus, which regulates the growth and fate of the cellular components of bone. The processes of mechano-sensory and -transduction towards bone formation have been well studied mainly in vivo as well as in two-dimensional (2D) dynamic cell culture platforms, which elucidated mechanically induced osteogenesis starting with anabolic responses, such as production of nitrogen oxide and prostaglandins followed by the activation of canonical Wnt signaling, upon mechanosensation. The knowledge has been now translated into regenerative medicine, particularly into the field of bone tissue engineering, where multipotent stem cells are combined with three-dimensional (3D) scaffolding biomaterials to produce transplantable constructs for bone regeneration. In the presence of 3D scaffolds, the importance of suitable dynamic cell culture platforms increases further not only to improve mass transfer inside the scaffolds but to provide appropriate biophysical cues to guide cell fate. In principle, the concept of dynamic cell culture platforms is rooted to bone mechanobiology. Therefore, this review primarily focuses on biophysical environment in bone and its translation into dynamic cell culture platforms commonly used for 2D and 3D cell expansion, including their advancement, challenges, and future perspectives. Additionally, it provides the literature review of recent empirical studies using 2D and 3D flow-based dynamic cell culture systems for bone tissue engineering. Research Network of Computational and Structural Biotechnology 2023-08-17 /pmc/articles/PMC10498129/ /pubmed/37711188 http://dx.doi.org/10.1016/j.csbj.2023.08.008 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review Article
Yamada, Shuntaro
Ockermann, Philipp Niklas
Schwarz, Thomas
Mustafa, Kamal
Hansmann, Jan
Translation of biophysical environment in bone into dynamic cell culture under flow for bone tissue engineering
title Translation of biophysical environment in bone into dynamic cell culture under flow for bone tissue engineering
title_full Translation of biophysical environment in bone into dynamic cell culture under flow for bone tissue engineering
title_fullStr Translation of biophysical environment in bone into dynamic cell culture under flow for bone tissue engineering
title_full_unstemmed Translation of biophysical environment in bone into dynamic cell culture under flow for bone tissue engineering
title_short Translation of biophysical environment in bone into dynamic cell culture under flow for bone tissue engineering
title_sort translation of biophysical environment in bone into dynamic cell culture under flow for bone tissue engineering
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10498129/
https://www.ncbi.nlm.nih.gov/pubmed/37711188
http://dx.doi.org/10.1016/j.csbj.2023.08.008
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