Cargando…

Sin3a associated protein 130 kDa, sap130, plays an evolutionary conserved role in zebrafish heart development

Hypoplastic left heart syndrome (HLHS) is a congenital heart disease where the left ventricle is reduced in size. A forward genetic screen in mice identified SIN3A associated protein 130 kDa (Sap130), part of the chromatin modifying SIN3A/HDAC complex, as a gene contributing to the etiology of HLHS....

Descripción completa

Detalles Bibliográficos
Autores principales: DeMoya, Ricardo A., Forman-Rubinsky, Rachel E., Fontaine, Deon, Shin, Joseph, Watkins, Simon C., Lo, Cecilia W., Tsang, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10498550/
https://www.ncbi.nlm.nih.gov/pubmed/37711853
http://dx.doi.org/10.3389/fcell.2023.1197109
_version_ 1785105544448573440
author DeMoya, Ricardo A.
Forman-Rubinsky, Rachel E.
Fontaine, Deon
Shin, Joseph
Watkins, Simon C.
Lo, Cecilia W.
Tsang, Michael
author_facet DeMoya, Ricardo A.
Forman-Rubinsky, Rachel E.
Fontaine, Deon
Shin, Joseph
Watkins, Simon C.
Lo, Cecilia W.
Tsang, Michael
author_sort DeMoya, Ricardo A.
collection PubMed
description Hypoplastic left heart syndrome (HLHS) is a congenital heart disease where the left ventricle is reduced in size. A forward genetic screen in mice identified SIN3A associated protein 130 kDa (Sap130), part of the chromatin modifying SIN3A/HDAC complex, as a gene contributing to the etiology of HLHS. Here, we report the role of zebrafish sap130 genes in heart development. Loss of sap130a, one of two Sap130 orthologs, resulted in smaller ventricle size, a phenotype reminiscent to the hypoplastic left ventricle in mice. While cardiac progenitors were normal during somitogenesis, diminution of the ventricle size suggest the Second Heart Field (SHF) was the source of the defect. To explore the role of sap130a in gene regulation, transcriptome profiling was performed after the heart tube formation to identify candidate pathways and genes responsible for the small ventricle phenotype. Genes involved in cardiac differentiation and cardiac function were dysregulated in sap130a, but not in sap130b mutants. Confocal light sheet analysis measured deficits in cardiac output in MZsap130a supporting the notion that cardiomyocyte maturation was disrupted. Lineage tracing experiments revealed a significant reduction of SHF cells in the ventricle that resulted in increased outflow tract size. These data suggest that sap130a is involved in cardiogenesis via regulating the accretion of SHF cells to the growing ventricle and in their subsequent maturation for cardiac function. Further, genetic studies revealed an interaction between hdac1 and sap130a, in the incidence of small ventricles. These studies highlight the conserved role of Sap130a and Hdac1 in zebrafish cardiogenesis.
format Online
Article
Text
id pubmed-10498550
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-104985502023-09-14 Sin3a associated protein 130 kDa, sap130, plays an evolutionary conserved role in zebrafish heart development DeMoya, Ricardo A. Forman-Rubinsky, Rachel E. Fontaine, Deon Shin, Joseph Watkins, Simon C. Lo, Cecilia W. Tsang, Michael Front Cell Dev Biol Cell and Developmental Biology Hypoplastic left heart syndrome (HLHS) is a congenital heart disease where the left ventricle is reduced in size. A forward genetic screen in mice identified SIN3A associated protein 130 kDa (Sap130), part of the chromatin modifying SIN3A/HDAC complex, as a gene contributing to the etiology of HLHS. Here, we report the role of zebrafish sap130 genes in heart development. Loss of sap130a, one of two Sap130 orthologs, resulted in smaller ventricle size, a phenotype reminiscent to the hypoplastic left ventricle in mice. While cardiac progenitors were normal during somitogenesis, diminution of the ventricle size suggest the Second Heart Field (SHF) was the source of the defect. To explore the role of sap130a in gene regulation, transcriptome profiling was performed after the heart tube formation to identify candidate pathways and genes responsible for the small ventricle phenotype. Genes involved in cardiac differentiation and cardiac function were dysregulated in sap130a, but not in sap130b mutants. Confocal light sheet analysis measured deficits in cardiac output in MZsap130a supporting the notion that cardiomyocyte maturation was disrupted. Lineage tracing experiments revealed a significant reduction of SHF cells in the ventricle that resulted in increased outflow tract size. These data suggest that sap130a is involved in cardiogenesis via regulating the accretion of SHF cells to the growing ventricle and in their subsequent maturation for cardiac function. Further, genetic studies revealed an interaction between hdac1 and sap130a, in the incidence of small ventricles. These studies highlight the conserved role of Sap130a and Hdac1 in zebrafish cardiogenesis. Frontiers Media S.A. 2023-08-30 /pmc/articles/PMC10498550/ /pubmed/37711853 http://dx.doi.org/10.3389/fcell.2023.1197109 Text en Copyright © 2023 DeMoya, Forman-Rubinsky, Fontaine, Shin, Watkins, Lo and Tsang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
DeMoya, Ricardo A.
Forman-Rubinsky, Rachel E.
Fontaine, Deon
Shin, Joseph
Watkins, Simon C.
Lo, Cecilia W.
Tsang, Michael
Sin3a associated protein 130 kDa, sap130, plays an evolutionary conserved role in zebrafish heart development
title Sin3a associated protein 130 kDa, sap130, plays an evolutionary conserved role in zebrafish heart development
title_full Sin3a associated protein 130 kDa, sap130, plays an evolutionary conserved role in zebrafish heart development
title_fullStr Sin3a associated protein 130 kDa, sap130, plays an evolutionary conserved role in zebrafish heart development
title_full_unstemmed Sin3a associated protein 130 kDa, sap130, plays an evolutionary conserved role in zebrafish heart development
title_short Sin3a associated protein 130 kDa, sap130, plays an evolutionary conserved role in zebrafish heart development
title_sort sin3a associated protein 130 kda, sap130, plays an evolutionary conserved role in zebrafish heart development
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10498550/
https://www.ncbi.nlm.nih.gov/pubmed/37711853
http://dx.doi.org/10.3389/fcell.2023.1197109
work_keys_str_mv AT demoyaricardoa sin3aassociatedprotein130kdasap130playsanevolutionaryconservedroleinzebrafishheartdevelopment
AT formanrubinskyrachele sin3aassociatedprotein130kdasap130playsanevolutionaryconservedroleinzebrafishheartdevelopment
AT fontainedeon sin3aassociatedprotein130kdasap130playsanevolutionaryconservedroleinzebrafishheartdevelopment
AT shinjoseph sin3aassociatedprotein130kdasap130playsanevolutionaryconservedroleinzebrafishheartdevelopment
AT watkinssimonc sin3aassociatedprotein130kdasap130playsanevolutionaryconservedroleinzebrafishheartdevelopment
AT loceciliaw sin3aassociatedprotein130kdasap130playsanevolutionaryconservedroleinzebrafishheartdevelopment
AT tsangmichael sin3aassociatedprotein130kdasap130playsanevolutionaryconservedroleinzebrafishheartdevelopment