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PD98059 protects SH-SY5Y cells against oxidative stress in oxygen–glucose deprivation/reperfusion

Mitochondria play a key role in the cerebral ischemia-reperfusion injury. Although the extracellular signal-regulated kinase 1/2 inhibitor PD98059 (PD) is a selective and reversible flavonoid that can protect the mitochondria in a rat model of cardiac arrest/cardiopulmonary resuscitation, its role r...

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Autores principales: Zhuge, Xiang-Zhen, Hu, Wan-Xiang, Liu, Yu-Mei, Jiang, Chang-Yue, Zhang, Xiao-Hua, Chen, Meng-Hua, Xie, Lu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: De Gruyter 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10500637/
https://www.ncbi.nlm.nih.gov/pubmed/37719747
http://dx.doi.org/10.1515/tnsci-2022-0300
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author Zhuge, Xiang-Zhen
Hu, Wan-Xiang
Liu, Yu-Mei
Jiang, Chang-Yue
Zhang, Xiao-Hua
Chen, Meng-Hua
Xie, Lu
author_facet Zhuge, Xiang-Zhen
Hu, Wan-Xiang
Liu, Yu-Mei
Jiang, Chang-Yue
Zhang, Xiao-Hua
Chen, Meng-Hua
Xie, Lu
author_sort Zhuge, Xiang-Zhen
collection PubMed
description Mitochondria play a key role in the cerebral ischemia-reperfusion injury. Although the extracellular signal-regulated kinase 1/2 inhibitor PD98059 (PD) is a selective and reversible flavonoid that can protect the mitochondria in a rat model of cardiac arrest/cardiopulmonary resuscitation, its role requires further confirmation. In this study, we investigated whether PD could maintain mitochondrial homeostasis and decrease reactive oxygen species (ROS) production in neuroblastoma (SH-SY5Y) cells exposed to oxygen–glucose deprivation/reperfusion (OGD/R). PD improved the mitochondrial morphology and function, reversed the increase in ROS production and cell apoptosis, and reduced total-superoxide dismutase and Mn-superoxide dismutase activities induced by OGD/R. PD decreases ROS production and improves mitochondrial morphology and function, protecting SH-SY5Y cells against OGD/R-induced injury.
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spelling pubmed-105006372023-09-15 PD98059 protects SH-SY5Y cells against oxidative stress in oxygen–glucose deprivation/reperfusion Zhuge, Xiang-Zhen Hu, Wan-Xiang Liu, Yu-Mei Jiang, Chang-Yue Zhang, Xiao-Hua Chen, Meng-Hua Xie, Lu Transl Neurosci Research Article Mitochondria play a key role in the cerebral ischemia-reperfusion injury. Although the extracellular signal-regulated kinase 1/2 inhibitor PD98059 (PD) is a selective and reversible flavonoid that can protect the mitochondria in a rat model of cardiac arrest/cardiopulmonary resuscitation, its role requires further confirmation. In this study, we investigated whether PD could maintain mitochondrial homeostasis and decrease reactive oxygen species (ROS) production in neuroblastoma (SH-SY5Y) cells exposed to oxygen–glucose deprivation/reperfusion (OGD/R). PD improved the mitochondrial morphology and function, reversed the increase in ROS production and cell apoptosis, and reduced total-superoxide dismutase and Mn-superoxide dismutase activities induced by OGD/R. PD decreases ROS production and improves mitochondrial morphology and function, protecting SH-SY5Y cells against OGD/R-induced injury. De Gruyter 2023-09-06 /pmc/articles/PMC10500637/ /pubmed/37719747 http://dx.doi.org/10.1515/tnsci-2022-0300 Text en © 2023 the author(s), published by De Gruyter https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License.
spellingShingle Research Article
Zhuge, Xiang-Zhen
Hu, Wan-Xiang
Liu, Yu-Mei
Jiang, Chang-Yue
Zhang, Xiao-Hua
Chen, Meng-Hua
Xie, Lu
PD98059 protects SH-SY5Y cells against oxidative stress in oxygen–glucose deprivation/reperfusion
title PD98059 protects SH-SY5Y cells against oxidative stress in oxygen–glucose deprivation/reperfusion
title_full PD98059 protects SH-SY5Y cells against oxidative stress in oxygen–glucose deprivation/reperfusion
title_fullStr PD98059 protects SH-SY5Y cells against oxidative stress in oxygen–glucose deprivation/reperfusion
title_full_unstemmed PD98059 protects SH-SY5Y cells against oxidative stress in oxygen–glucose deprivation/reperfusion
title_short PD98059 protects SH-SY5Y cells against oxidative stress in oxygen–glucose deprivation/reperfusion
title_sort pd98059 protects sh-sy5y cells against oxidative stress in oxygen–glucose deprivation/reperfusion
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10500637/
https://www.ncbi.nlm.nih.gov/pubmed/37719747
http://dx.doi.org/10.1515/tnsci-2022-0300
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