Cargando…
PD98059 protects SH-SY5Y cells against oxidative stress in oxygen–glucose deprivation/reperfusion
Mitochondria play a key role in the cerebral ischemia-reperfusion injury. Although the extracellular signal-regulated kinase 1/2 inhibitor PD98059 (PD) is a selective and reversible flavonoid that can protect the mitochondria in a rat model of cardiac arrest/cardiopulmonary resuscitation, its role r...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
De Gruyter
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10500637/ https://www.ncbi.nlm.nih.gov/pubmed/37719747 http://dx.doi.org/10.1515/tnsci-2022-0300 |
_version_ | 1785105948724953088 |
---|---|
author | Zhuge, Xiang-Zhen Hu, Wan-Xiang Liu, Yu-Mei Jiang, Chang-Yue Zhang, Xiao-Hua Chen, Meng-Hua Xie, Lu |
author_facet | Zhuge, Xiang-Zhen Hu, Wan-Xiang Liu, Yu-Mei Jiang, Chang-Yue Zhang, Xiao-Hua Chen, Meng-Hua Xie, Lu |
author_sort | Zhuge, Xiang-Zhen |
collection | PubMed |
description | Mitochondria play a key role in the cerebral ischemia-reperfusion injury. Although the extracellular signal-regulated kinase 1/2 inhibitor PD98059 (PD) is a selective and reversible flavonoid that can protect the mitochondria in a rat model of cardiac arrest/cardiopulmonary resuscitation, its role requires further confirmation. In this study, we investigated whether PD could maintain mitochondrial homeostasis and decrease reactive oxygen species (ROS) production in neuroblastoma (SH-SY5Y) cells exposed to oxygen–glucose deprivation/reperfusion (OGD/R). PD improved the mitochondrial morphology and function, reversed the increase in ROS production and cell apoptosis, and reduced total-superoxide dismutase and Mn-superoxide dismutase activities induced by OGD/R. PD decreases ROS production and improves mitochondrial morphology and function, protecting SH-SY5Y cells against OGD/R-induced injury. |
format | Online Article Text |
id | pubmed-10500637 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | De Gruyter |
record_format | MEDLINE/PubMed |
spelling | pubmed-105006372023-09-15 PD98059 protects SH-SY5Y cells against oxidative stress in oxygen–glucose deprivation/reperfusion Zhuge, Xiang-Zhen Hu, Wan-Xiang Liu, Yu-Mei Jiang, Chang-Yue Zhang, Xiao-Hua Chen, Meng-Hua Xie, Lu Transl Neurosci Research Article Mitochondria play a key role in the cerebral ischemia-reperfusion injury. Although the extracellular signal-regulated kinase 1/2 inhibitor PD98059 (PD) is a selective and reversible flavonoid that can protect the mitochondria in a rat model of cardiac arrest/cardiopulmonary resuscitation, its role requires further confirmation. In this study, we investigated whether PD could maintain mitochondrial homeostasis and decrease reactive oxygen species (ROS) production in neuroblastoma (SH-SY5Y) cells exposed to oxygen–glucose deprivation/reperfusion (OGD/R). PD improved the mitochondrial morphology and function, reversed the increase in ROS production and cell apoptosis, and reduced total-superoxide dismutase and Mn-superoxide dismutase activities induced by OGD/R. PD decreases ROS production and improves mitochondrial morphology and function, protecting SH-SY5Y cells against OGD/R-induced injury. De Gruyter 2023-09-06 /pmc/articles/PMC10500637/ /pubmed/37719747 http://dx.doi.org/10.1515/tnsci-2022-0300 Text en © 2023 the author(s), published by De Gruyter https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. |
spellingShingle | Research Article Zhuge, Xiang-Zhen Hu, Wan-Xiang Liu, Yu-Mei Jiang, Chang-Yue Zhang, Xiao-Hua Chen, Meng-Hua Xie, Lu PD98059 protects SH-SY5Y cells against oxidative stress in oxygen–glucose deprivation/reperfusion |
title | PD98059 protects SH-SY5Y cells against oxidative stress in oxygen–glucose deprivation/reperfusion |
title_full | PD98059 protects SH-SY5Y cells against oxidative stress in oxygen–glucose deprivation/reperfusion |
title_fullStr | PD98059 protects SH-SY5Y cells against oxidative stress in oxygen–glucose deprivation/reperfusion |
title_full_unstemmed | PD98059 protects SH-SY5Y cells against oxidative stress in oxygen–glucose deprivation/reperfusion |
title_short | PD98059 protects SH-SY5Y cells against oxidative stress in oxygen–glucose deprivation/reperfusion |
title_sort | pd98059 protects sh-sy5y cells against oxidative stress in oxygen–glucose deprivation/reperfusion |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10500637/ https://www.ncbi.nlm.nih.gov/pubmed/37719747 http://dx.doi.org/10.1515/tnsci-2022-0300 |
work_keys_str_mv | AT zhugexiangzhen pd98059protectsshsy5ycellsagainstoxidativestressinoxygenglucosedeprivationreperfusion AT huwanxiang pd98059protectsshsy5ycellsagainstoxidativestressinoxygenglucosedeprivationreperfusion AT liuyumei pd98059protectsshsy5ycellsagainstoxidativestressinoxygenglucosedeprivationreperfusion AT jiangchangyue pd98059protectsshsy5ycellsagainstoxidativestressinoxygenglucosedeprivationreperfusion AT zhangxiaohua pd98059protectsshsy5ycellsagainstoxidativestressinoxygenglucosedeprivationreperfusion AT chenmenghua pd98059protectsshsy5ycellsagainstoxidativestressinoxygenglucosedeprivationreperfusion AT xielu pd98059protectsshsy5ycellsagainstoxidativestressinoxygenglucosedeprivationreperfusion |