Endoplasmic Reticulum Isolation: An Optimized Approach into Cells and Mouse Liver Fractionation

The subfractionation of the endoplasmic reticulum (ER) is a widely used technique in cell biology. However, current protocols present limitations such as low yield, the use of large number of dishes, and contamination with other organelles. Here, we describe an improved method for ER subfractionatio...

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Detalles Bibliográficos
Autores principales: Leiro, Marc, Ventura, Raúl, Rojo-Querol, Nil, Hernández-Alvarez, María Isabel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10501922/
https://www.ncbi.nlm.nih.gov/pubmed/37719073
http://dx.doi.org/10.21769/BioProtoc.4803

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10501922/
https://www.ncbi.nlm.nih.gov/pubmed/37719073
http://dx.doi.org/10.21769/BioProtoc.4803

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