Cargando…
Endoplasmic Reticulum Isolation: An Optimized Approach into Cells and Mouse Liver Fractionation
The subfractionation of the endoplasmic reticulum (ER) is a widely used technique in cell biology. However, current protocols present limitations such as low yield, the use of large number of dishes, and contamination with other organelles. Here, we describe an improved method for ER subfractionatio...
Autores principales: | Leiro, Marc, Ventura, Raúl, Rojo-Querol, Nil, Hernández-Alvarez, María Isabel |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bio-Protocol
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10501922/ https://www.ncbi.nlm.nih.gov/pubmed/37719073 http://dx.doi.org/10.21769/BioProtoc.4803 |
Ejemplares similares
-
Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes
por: Devulapally, Praneeth Reddy, et al.
Publicado: (2018) -
Two fractions of rough endoplasmic reticulum from rat liver. I. Recovery of rapidly sedimenting endoplasmic reticulum in association with mitochondria
Publicado: (1977) -
A high-throughput screening of genes that encode proteins transported into the endoplasmic reticulum in mammalian cells
por: Ozawa, Takeaki, et al.
Publicado: (2005) -
Glycosyltransferase activities in Golgi complex and endoplasmic reticulum fractions isolated from African trypanosomes
Publicado: (1984) -
Endoplasmic reticulum protein 5 attenuates platelet endoplasmic reticulum stress and secretion in a mouse model
por: Lay, Angelina J., et al.
Publicado: (2022)