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gRNA-SeqRET: a universal tool for targeted and genome-scale gRNA design and sequence extraction for prokaryotes and eukaryotes

High-throughput genetic screening is frequently employed to rapidly associate gene with phenotype and establish sequence-function relationships. With the advent of CRISPR technology, and the ability to functionally interrogate previously genetically recalcitrant organisms, non-model organisms can be...

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Detalles Bibliográficos
Autores principales: Simirenko, Lisa, Cheng, Jan-Fang, Blaby, Ian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10502169/
https://www.ncbi.nlm.nih.gov/pubmed/37720317
http://dx.doi.org/10.3389/fbioe.2023.1217811