A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets

Streptococcus pyogenes Cas9 (SpCas9) has been employed as a genome engineering tool with a promising potential within therapeutics. However, its off-target effects present major safety concerns for applications requiring high specificity. Approaches developed to date to mitigate this effect, includi...

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Detalles Bibliográficos
Autores principales: Kulcsár, Péter István, Tálas, András, Ligeti, Zoltán, Tóth, Eszter, Rakvács, Zsófia, Bartos, Zsuzsa, Krausz, Sarah Laura, Welker, Ágnes, Végi, Vanessza Laura, Huszár, Krisztina, Welker, Ervin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505190/
https://www.ncbi.nlm.nih.gov/pubmed/37717069
http://dx.doi.org/10.1038/s41467-023-41393-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10505190/
https://www.ncbi.nlm.nih.gov/pubmed/37717069
http://dx.doi.org/10.1038/s41467-023-41393-5

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