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Loss of ATP-sensitive channel expression and function decreases opioid sensitivity in a mouse model of type 2 diabetes

During diabetes, β-cell dysfunction due to loss of potassium channels sensitive to ATP, known as K(ATP) channels occurs progressively over time contributing to hyperglycemia. K(ATP) channels are additionally present in the central and peripheral nervous systems and are downstream targets of opioid r...

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Autores principales: Fisher, Cole, Johnson, Kayla, Moore, Madelyn, Sadrati, Amir, Janecek, Jody L., Graham, Melanie L., Klein, Amanda H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10508758/
https://www.ncbi.nlm.nih.gov/pubmed/37732180
http://dx.doi.org/10.1101/2023.09.06.556526
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author Fisher, Cole
Johnson, Kayla
Moore, Madelyn
Sadrati, Amir
Janecek, Jody L.
Graham, Melanie L.
Klein, Amanda H.
author_facet Fisher, Cole
Johnson, Kayla
Moore, Madelyn
Sadrati, Amir
Janecek, Jody L.
Graham, Melanie L.
Klein, Amanda H.
author_sort Fisher, Cole
collection PubMed
description During diabetes, β-cell dysfunction due to loss of potassium channels sensitive to ATP, known as K(ATP) channels occurs progressively over time contributing to hyperglycemia. K(ATP) channels are additionally present in the central and peripheral nervous systems and are downstream targets of opioid receptor signaling. The aim of this study is to investigate if K(ATP) channel expression or activity in the nervous system changes in diabetic mice and if morphine antinociception changes in mice fed a high fat diet (HFD) for 16 weeks compared to controls. Mechanical thresholds were also monitored before and after administration of glyburide or nateglinide, K(ATP) channel antagonists, for four weeks. HFD mice have decreased antinociception to systemic morphine, which is exacerbated after systemic treatment with glyburide or nateglinide. HFD mice also have lower rotarod scores, decreased mobility in an open field test, and lower burrowing behavior compared to their control diet counterparts, which is unaffected by K(ATP) channel antagonist delivery. Expression of K(ATP) channel subunits, Kcnj11 (Kir6.2) and Abcc8 (SUR1), were decreased in the peripheral and central nervous system in HFD mice, which is significantly correlated with baseline paw withdrawal thresholds. Upregulation of SUR1 through an adenovirus delivered intrathecally increased morphine antinociception in HFD mice, whereas Kir6.2 upregulation improved morphine antinociception only marginally. Perspective: This article presents the potential link between K(ATP) channel function and neuropathy during diabetes. There is a need for increased knowledge in how diabetes affects structural and molecular changes in the nervous system to lead to the progression of chronic pain and sensory issues.
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spelling pubmed-105087582023-09-20 Loss of ATP-sensitive channel expression and function decreases opioid sensitivity in a mouse model of type 2 diabetes Fisher, Cole Johnson, Kayla Moore, Madelyn Sadrati, Amir Janecek, Jody L. Graham, Melanie L. Klein, Amanda H. bioRxiv Article During diabetes, β-cell dysfunction due to loss of potassium channels sensitive to ATP, known as K(ATP) channels occurs progressively over time contributing to hyperglycemia. K(ATP) channels are additionally present in the central and peripheral nervous systems and are downstream targets of opioid receptor signaling. The aim of this study is to investigate if K(ATP) channel expression or activity in the nervous system changes in diabetic mice and if morphine antinociception changes in mice fed a high fat diet (HFD) for 16 weeks compared to controls. Mechanical thresholds were also monitored before and after administration of glyburide or nateglinide, K(ATP) channel antagonists, for four weeks. HFD mice have decreased antinociception to systemic morphine, which is exacerbated after systemic treatment with glyburide or nateglinide. HFD mice also have lower rotarod scores, decreased mobility in an open field test, and lower burrowing behavior compared to their control diet counterparts, which is unaffected by K(ATP) channel antagonist delivery. Expression of K(ATP) channel subunits, Kcnj11 (Kir6.2) and Abcc8 (SUR1), were decreased in the peripheral and central nervous system in HFD mice, which is significantly correlated with baseline paw withdrawal thresholds. Upregulation of SUR1 through an adenovirus delivered intrathecally increased morphine antinociception in HFD mice, whereas Kir6.2 upregulation improved morphine antinociception only marginally. Perspective: This article presents the potential link between K(ATP) channel function and neuropathy during diabetes. There is a need for increased knowledge in how diabetes affects structural and molecular changes in the nervous system to lead to the progression of chronic pain and sensory issues. Cold Spring Harbor Laboratory 2023-09-06 /pmc/articles/PMC10508758/ /pubmed/37732180 http://dx.doi.org/10.1101/2023.09.06.556526 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Fisher, Cole
Johnson, Kayla
Moore, Madelyn
Sadrati, Amir
Janecek, Jody L.
Graham, Melanie L.
Klein, Amanda H.
Loss of ATP-sensitive channel expression and function decreases opioid sensitivity in a mouse model of type 2 diabetes
title Loss of ATP-sensitive channel expression and function decreases opioid sensitivity in a mouse model of type 2 diabetes
title_full Loss of ATP-sensitive channel expression and function decreases opioid sensitivity in a mouse model of type 2 diabetes
title_fullStr Loss of ATP-sensitive channel expression and function decreases opioid sensitivity in a mouse model of type 2 diabetes
title_full_unstemmed Loss of ATP-sensitive channel expression and function decreases opioid sensitivity in a mouse model of type 2 diabetes
title_short Loss of ATP-sensitive channel expression and function decreases opioid sensitivity in a mouse model of type 2 diabetes
title_sort loss of atp-sensitive channel expression and function decreases opioid sensitivity in a mouse model of type 2 diabetes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10508758/
https://www.ncbi.nlm.nih.gov/pubmed/37732180
http://dx.doi.org/10.1101/2023.09.06.556526
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