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Joint genotypic and phenotypic outcome modeling improves base editing variant effect quantification

CRISPR base editing screens are powerful tools for studying disease-associated variants at scale. However, the efficiency and precision of base editing perturbations vary, confounding the assessment of variant-induced phenotypic effects. Here, we provide an integrated pipeline that improves the esti...

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Autores principales: Ryu, Jayoung, Barkal, Sam, Yu, Tian, Jankowiak, Martin, Zhou, Yunzhuo, Francoeur, Matthew, Phan, Quang Vinh, Li, Zhijian, Tognon, Manuel, Brown, Lara, Love, Michael I., Lettre, Guillaume, Ascher, David B., Cassa, Christopher A., Sherwood, Richard I., Pinello, Luca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10508837/
https://www.ncbi.nlm.nih.gov/pubmed/37732177
http://dx.doi.org/10.1101/2023.09.08.23295253
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author Ryu, Jayoung
Barkal, Sam
Yu, Tian
Jankowiak, Martin
Zhou, Yunzhuo
Francoeur, Matthew
Phan, Quang Vinh
Li, Zhijian
Tognon, Manuel
Brown, Lara
Love, Michael I.
Lettre, Guillaume
Ascher, David B.
Cassa, Christopher A.
Sherwood, Richard I.
Pinello, Luca
author_facet Ryu, Jayoung
Barkal, Sam
Yu, Tian
Jankowiak, Martin
Zhou, Yunzhuo
Francoeur, Matthew
Phan, Quang Vinh
Li, Zhijian
Tognon, Manuel
Brown, Lara
Love, Michael I.
Lettre, Guillaume
Ascher, David B.
Cassa, Christopher A.
Sherwood, Richard I.
Pinello, Luca
author_sort Ryu, Jayoung
collection PubMed
description CRISPR base editing screens are powerful tools for studying disease-associated variants at scale. However, the efficiency and precision of base editing perturbations vary, confounding the assessment of variant-induced phenotypic effects. Here, we provide an integrated pipeline that improves the estimation of variant impact in base editing screens. We perform high-throughput ABE8e-SpRY base editing screens with an integrated reporter construct to measure the editing efficiency and outcomes of each gRNA alongside their phenotypic consequences. We introduce BEAN, a Bayesian network that accounts for per-guide editing outcomes and target site chromatin accessibility to estimate variant impacts. We show this pipeline attains superior performance compared to existing tools in variant classification and effect size quantification. We use BEAN to pinpoint common variants that alter LDL uptake, implicating novel genes. Additionally, through saturation base editing of LDLR, we enable accurate quantitative prediction of the effects of missense variants on LDL-C levels, which aligns with measurements in UK Biobank individuals, and identify structural mechanisms underlying variant pathogenicity. This work provides a widely applicable approach to improve the power of base editor screens for disease-associated variant characterization.
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spelling pubmed-105088372023-09-20 Joint genotypic and phenotypic outcome modeling improves base editing variant effect quantification Ryu, Jayoung Barkal, Sam Yu, Tian Jankowiak, Martin Zhou, Yunzhuo Francoeur, Matthew Phan, Quang Vinh Li, Zhijian Tognon, Manuel Brown, Lara Love, Michael I. Lettre, Guillaume Ascher, David B. Cassa, Christopher A. Sherwood, Richard I. Pinello, Luca medRxiv Article CRISPR base editing screens are powerful tools for studying disease-associated variants at scale. However, the efficiency and precision of base editing perturbations vary, confounding the assessment of variant-induced phenotypic effects. Here, we provide an integrated pipeline that improves the estimation of variant impact in base editing screens. We perform high-throughput ABE8e-SpRY base editing screens with an integrated reporter construct to measure the editing efficiency and outcomes of each gRNA alongside their phenotypic consequences. We introduce BEAN, a Bayesian network that accounts for per-guide editing outcomes and target site chromatin accessibility to estimate variant impacts. We show this pipeline attains superior performance compared to existing tools in variant classification and effect size quantification. We use BEAN to pinpoint common variants that alter LDL uptake, implicating novel genes. Additionally, through saturation base editing of LDLR, we enable accurate quantitative prediction of the effects of missense variants on LDL-C levels, which aligns with measurements in UK Biobank individuals, and identify structural mechanisms underlying variant pathogenicity. This work provides a widely applicable approach to improve the power of base editor screens for disease-associated variant characterization. Cold Spring Harbor Laboratory 2023-09-10 /pmc/articles/PMC10508837/ /pubmed/37732177 http://dx.doi.org/10.1101/2023.09.08.23295253 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Ryu, Jayoung
Barkal, Sam
Yu, Tian
Jankowiak, Martin
Zhou, Yunzhuo
Francoeur, Matthew
Phan, Quang Vinh
Li, Zhijian
Tognon, Manuel
Brown, Lara
Love, Michael I.
Lettre, Guillaume
Ascher, David B.
Cassa, Christopher A.
Sherwood, Richard I.
Pinello, Luca
Joint genotypic and phenotypic outcome modeling improves base editing variant effect quantification
title Joint genotypic and phenotypic outcome modeling improves base editing variant effect quantification
title_full Joint genotypic and phenotypic outcome modeling improves base editing variant effect quantification
title_fullStr Joint genotypic and phenotypic outcome modeling improves base editing variant effect quantification
title_full_unstemmed Joint genotypic and phenotypic outcome modeling improves base editing variant effect quantification
title_short Joint genotypic and phenotypic outcome modeling improves base editing variant effect quantification
title_sort joint genotypic and phenotypic outcome modeling improves base editing variant effect quantification
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10508837/
https://www.ncbi.nlm.nih.gov/pubmed/37732177
http://dx.doi.org/10.1101/2023.09.08.23295253
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