Cargando…

Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damage

OBJECTIVE(S): Renal ischemia-reperfusion (I/R) is a vital health condition leading to acute kidney injury. Costunolide (COST) is an actively used molecule clinically for its anti-inflammatory, antioxidant, and immunomodulatory properties. In the present study, we searched for the possible protective...

Descripción completa

Detalles Bibliográficos
Autores principales: Güler, Mustafa Can, Akpinar, Erol, Tanyeli, Ayhan, Çomakli, Selim, Bayir, Yasin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510491/
https://www.ncbi.nlm.nih.gov/pubmed/37736519
http://dx.doi.org/10.22038/IJBMS.2023.71779.15596
_version_ 1785107979701321728
author Güler, Mustafa Can
Akpinar, Erol
Tanyeli, Ayhan
Çomakli, Selim
Bayir, Yasin
author_facet Güler, Mustafa Can
Akpinar, Erol
Tanyeli, Ayhan
Çomakli, Selim
Bayir, Yasin
author_sort Güler, Mustafa Can
collection PubMed
description OBJECTIVE(S): Renal ischemia-reperfusion (I/R) is a vital health condition leading to acute kidney injury. Costunolide (COST) is an actively used molecule clinically for its anti-inflammatory, antioxidant, and immunomodulatory properties. In the present study, we searched for the possible protective effects of COST against renal ischemia/reperfusion (I/R) injury in rats. MATERIALS AND METHODS: We established a renal I/R rat model. We divided forty rats into four groups: group I (sham), group II (I/R), group III (I/R+COST 5 mg/kg), and group IV (I/R+COST 10 mg/kg). We collected blood, kidney, and lung samples for analysis. RESULTS: COST administration performed anti-oxidant and anti-inflammatory activity by reducing oxidant parameters and proinflammatory cytokine levels. COST alleviated DNA damage through declining 8-hydroxydeoxyguanosine (8-OHdG) levels. In addition, COST diminished tubular damage and inflammation by reducing kidney injury molecule-1 (KIM-1) production. COST administration also ameliorated apoptosis and autophagy by decreasing caspase-3 and microtubule-associated protein light chain 3B (MAPLC3, LC3B) expression. CONCLUSION: COST demonstrated protective effects against renal I/R-induced injury.
format Online
Article
Text
id pubmed-10510491
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Mashhad University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-105104912023-09-21 Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damage Güler, Mustafa Can Akpinar, Erol Tanyeli, Ayhan Çomakli, Selim Bayir, Yasin Iran J Basic Med Sci Original Article OBJECTIVE(S): Renal ischemia-reperfusion (I/R) is a vital health condition leading to acute kidney injury. Costunolide (COST) is an actively used molecule clinically for its anti-inflammatory, antioxidant, and immunomodulatory properties. In the present study, we searched for the possible protective effects of COST against renal ischemia/reperfusion (I/R) injury in rats. MATERIALS AND METHODS: We established a renal I/R rat model. We divided forty rats into four groups: group I (sham), group II (I/R), group III (I/R+COST 5 mg/kg), and group IV (I/R+COST 10 mg/kg). We collected blood, kidney, and lung samples for analysis. RESULTS: COST administration performed anti-oxidant and anti-inflammatory activity by reducing oxidant parameters and proinflammatory cytokine levels. COST alleviated DNA damage through declining 8-hydroxydeoxyguanosine (8-OHdG) levels. In addition, COST diminished tubular damage and inflammation by reducing kidney injury molecule-1 (KIM-1) production. COST administration also ameliorated apoptosis and autophagy by decreasing caspase-3 and microtubule-associated protein light chain 3B (MAPLC3, LC3B) expression. CONCLUSION: COST demonstrated protective effects against renal I/R-induced injury. Mashhad University of Medical Sciences 2023 /pmc/articles/PMC10510491/ /pubmed/37736519 http://dx.doi.org/10.22038/IJBMS.2023.71779.15596 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Güler, Mustafa Can
Akpinar, Erol
Tanyeli, Ayhan
Çomakli, Selim
Bayir, Yasin
Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damage
title Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damage
title_full Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damage
title_fullStr Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damage
title_full_unstemmed Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damage
title_short Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damage
title_sort costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and dna damage
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510491/
https://www.ncbi.nlm.nih.gov/pubmed/37736519
http://dx.doi.org/10.22038/IJBMS.2023.71779.15596
work_keys_str_mv AT gulermustafacan costunolidepreventsrenalischemiareperfusioninjuryinratsbyreducingautophagyapoptosisinflammationanddnadamage
AT akpinarerol costunolidepreventsrenalischemiareperfusioninjuryinratsbyreducingautophagyapoptosisinflammationanddnadamage
AT tanyeliayhan costunolidepreventsrenalischemiareperfusioninjuryinratsbyreducingautophagyapoptosisinflammationanddnadamage
AT comakliselim costunolidepreventsrenalischemiareperfusioninjuryinratsbyreducingautophagyapoptosisinflammationanddnadamage
AT bayiryasin costunolidepreventsrenalischemiareperfusioninjuryinratsbyreducingautophagyapoptosisinflammationanddnadamage