Structural transitions upon guide RNA binding and their importance in Cas12g-mediated RNA cleavage

Cas12g is an endonuclease belonging to the type V RNA-guided CRISPR–Cas family. It is known for its ability to cleave RNA substrates using a conserved endonuclease active site located in the RuvC domain. In this study, we determined the crystal structure of apo-Cas12g, the cryo-EM structure of the C...

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Detalles Bibliográficos
Autores principales: Liu, Mengxi, Li, Zekai, Chen, Jing, Lin, Jinying, Lu, Qiuhua, Ye, Yangmiao, Zhang, Hongmin, Zhang, Bo, Ouyang, Songying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511118/
https://www.ncbi.nlm.nih.gov/pubmed/37729124
http://dx.doi.org/10.1371/journal.pgen.1010930

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511118/
https://www.ncbi.nlm.nih.gov/pubmed/37729124
http://dx.doi.org/10.1371/journal.pgen.1010930

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