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A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons
Here, we present a protocol for the generation of functional midbrain dopaminergic (mDA) neurons from human embryonic stem cells (hESCs), which mimics the development of the human ventral midbrain. We describe steps for hESC proliferation, induction of mDA progenitors, freezing stocks of mDA progeni...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511858/ https://www.ncbi.nlm.nih.gov/pubmed/37310863 http://dx.doi.org/10.1016/j.xpro.2023.102355 |