A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons

Here, we present a protocol for the generation of functional midbrain dopaminergic (mDA) neurons from human embryonic stem cells (hESCs), which mimics the development of the human ventral midbrain. We describe steps for hESC proliferation, induction of mDA progenitors, freezing stocks of mDA progeni...

Descripción completa

Detalles Bibliográficos
Autores principales: Nishimura, Kaneyasu, Ásgrímsdóttir, Emilía Sif, Yang, Shanzheng, Arenas, Ernest
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511858/
https://www.ncbi.nlm.nih.gov/pubmed/37310863
http://dx.doi.org/10.1016/j.xpro.2023.102355
Descripción
Sumario:Here, we present a protocol for the generation of functional midbrain dopaminergic (mDA) neurons from human embryonic stem cells (hESCs), which mimics the development of the human ventral midbrain. We describe steps for hESC proliferation, induction of mDA progenitors, freezing stocks of mDA progenitors as an intermediate starting point to reduce the time to make mDA neurons, and maturation of mDA neurons. The entire protocol is feeder-free and uses chemically defined materials. For complete details on the use and execution of this protocol, please refer to Nishimura et al. (2023).(1)

Ejemplares similares