A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons

Here, we present a protocol for the generation of functional midbrain dopaminergic (mDA) neurons from human embryonic stem cells (hESCs), which mimics the development of the human ventral midbrain. We describe steps for hESC proliferation, induction of mDA progenitors, freezing stocks of mDA progeni...

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Autores principales: Nishimura, Kaneyasu, Ásgrímsdóttir, Emilía Sif, Yang, Shanzheng, Arenas, Ernest
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511858/
https://www.ncbi.nlm.nih.gov/pubmed/37310863
http://dx.doi.org/10.1016/j.xpro.2023.102355
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author Nishimura, Kaneyasu
Ásgrímsdóttir, Emilía Sif
Yang, Shanzheng
Arenas, Ernest
author_facet Nishimura, Kaneyasu
Ásgrímsdóttir, Emilía Sif
Yang, Shanzheng
Arenas, Ernest
author_sort Nishimura, Kaneyasu
collection PubMed
description Here, we present a protocol for the generation of functional midbrain dopaminergic (mDA) neurons from human embryonic stem cells (hESCs), which mimics the development of the human ventral midbrain. We describe steps for hESC proliferation, induction of mDA progenitors, freezing stocks of mDA progenitors as an intermediate starting point to reduce the time to make mDA neurons, and maturation of mDA neurons. The entire protocol is feeder-free and uses chemically defined materials. For complete details on the use and execution of this protocol, please refer to Nishimura et al. (2023).(1)
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spelling pubmed-105118582023-09-22 A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons Nishimura, Kaneyasu Ásgrímsdóttir, Emilía Sif Yang, Shanzheng Arenas, Ernest STAR Protoc Protocol Here, we present a protocol for the generation of functional midbrain dopaminergic (mDA) neurons from human embryonic stem cells (hESCs), which mimics the development of the human ventral midbrain. We describe steps for hESC proliferation, induction of mDA progenitors, freezing stocks of mDA progenitors as an intermediate starting point to reduce the time to make mDA neurons, and maturation of mDA neurons. The entire protocol is feeder-free and uses chemically defined materials. For complete details on the use and execution of this protocol, please refer to Nishimura et al. (2023).(1) Elsevier 2023-06-12 /pmc/articles/PMC10511858/ /pubmed/37310863 http://dx.doi.org/10.1016/j.xpro.2023.102355 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Nishimura, Kaneyasu
Ásgrímsdóttir, Emilía Sif
Yang, Shanzheng
Arenas, Ernest
A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons
title A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons
title_full A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons
title_fullStr A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons
title_full_unstemmed A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons
title_short A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons
title_sort protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511858/
https://www.ncbi.nlm.nih.gov/pubmed/37310863
http://dx.doi.org/10.1016/j.xpro.2023.102355
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