A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons

Here, we present a protocol for the generation of functional midbrain dopaminergic (mDA) neurons from human embryonic stem cells (hESCs), which mimics the development of the human ventral midbrain. We describe steps for hESC proliferation, induction of mDA progenitors, freezing stocks of mDA progeni...

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Detalles Bibliográficos
Autores principales: Nishimura, Kaneyasu, Ásgrímsdóttir, Emilía Sif, Yang, Shanzheng, Arenas, Ernest
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511858/
https://www.ncbi.nlm.nih.gov/pubmed/37310863
http://dx.doi.org/10.1016/j.xpro.2023.102355

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