Engineering the sialome of mammalian cells with sialic acid mimetics

Mammalian glycans show a diversity in sialic acid capping, constituting the sialome. Sialic acids can be extensively modified chemically, yielding sialic acid mimetics (SAMs). Here, we present a protocol for detecting and quantifying incorporative SAMs using microscopy and flow cytometry, respective...

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Detalles Bibliográficos
Autores principales: Hornikx, Daniël L.A.H., Visser, Eline A., Psomiadou, Venetia, Büll, Christian, Boltje, Thomas J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511862/
https://www.ncbi.nlm.nih.gov/pubmed/37389993
http://dx.doi.org/10.1016/j.xpro.2023.102330

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511862/
https://www.ncbi.nlm.nih.gov/pubmed/37389993
http://dx.doi.org/10.1016/j.xpro.2023.102330

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