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Engineering the sialome of mammalian cells with sialic acid mimetics
Mammalian glycans show a diversity in sialic acid capping, constituting the sialome. Sialic acids can be extensively modified chemically, yielding sialic acid mimetics (SAMs). Here, we present a protocol for detecting and quantifying incorporative SAMs using microscopy and flow cytometry, respective...
Autores principales: | Hornikx, Daniël L.A.H., Visser, Eline A., Psomiadou, Venetia, Büll, Christian, Boltje, Thomas J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511862/ https://www.ncbi.nlm.nih.gov/pubmed/37389993 http://dx.doi.org/10.1016/j.xpro.2023.102330 |
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