Protocols for transgenesis at a safe harbor site in the Xenopus laevis genome using CRISPR-Cas9

We have established a new transgenesis protocol based on CRISPR-Cas9, “New and Easy XenopusTransgenesis (NEXTrans),” and identified a novel safe harbor site in African clawed frogs, Xenopus laevis. We describe steps in detail for the construction of NEXTrans plasmid and guide RNA, CRISPR-Cas9-mediat...

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Detalles Bibliográficos
Autores principales: Shibata, Yuki, Okumura, Akinori, Mochii, Makoto, Suzuki, Ken-ichi T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511863/
https://www.ncbi.nlm.nih.gov/pubmed/37389994
http://dx.doi.org/10.1016/j.xpro.2023.102382

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511863/
https://www.ncbi.nlm.nih.gov/pubmed/37389994
http://dx.doi.org/10.1016/j.xpro.2023.102382

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