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Protocols for transgenesis at a safe harbor site in the Xenopus laevis genome using CRISPR-Cas9

We have established a new transgenesis protocol based on CRISPR-Cas9, “New and Easy XenopusTransgenesis (NEXTrans),” and identified a novel safe harbor site in African clawed frogs, Xenopus laevis. We describe steps in detail for the construction of NEXTrans plasmid and guide RNA, CRISPR-Cas9-mediat...

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Detalles Bibliográficos
Autores principales:	Shibata, Yuki, Okumura, Akinori, Mochii, Makoto, Suzuki, Ken-ichi T.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511863/ https://www.ncbi.nlm.nih.gov/pubmed/37389994 http://dx.doi.org/10.1016/j.xpro.2023.102382

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