Three methods for examining the de novo proteome of microglia using BONCAT bioorthogonal labeling and FUNCAT click chemistry

Bioorthogonal labeling and click chemistry techniques allow the detailed examination of cellular physiology through tagging and visualizing newly synthesized proteins. Here, we describe three methods applying bioorthogonal non-canonical amino acid tagging and fluorescent non-canonical amino acid tag...

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Detalles Bibliográficos
Autores principales: Carlisle, Alison Keolani, Götz, Jürgen, Bodea, Liviu-Gabriel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511912/
https://www.ncbi.nlm.nih.gov/pubmed/37432857
http://dx.doi.org/10.1016/j.xpro.2023.102418

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511912/
https://www.ncbi.nlm.nih.gov/pubmed/37432857
http://dx.doi.org/10.1016/j.xpro.2023.102418

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