Protocol to dissociate and isolate wide-diversity single cells by density gradient centrifugation from human hepatoblastoma tissue

Single-cell transcriptome sequencing can characterize various cell types in human liver tissue and facilitate understanding of hepatoblastoma heterogeneity. Here, we present a protocol for isolating hepatocytes and immune cells from human hepatoblastoma samples with high viability. We describe steps...

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Detalles Bibliográficos
Autores principales: Zhou, Xianchao, Meng, Mei, Wu, Yifan, Gao, Rui, Shan, Yuhua, Gu, Song, He, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511933/
https://www.ncbi.nlm.nih.gov/pubmed/37459235
http://dx.doi.org/10.1016/j.xpro.2023.102449

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511933/
https://www.ncbi.nlm.nih.gov/pubmed/37459235
http://dx.doi.org/10.1016/j.xpro.2023.102449

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