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An experimental comparison between primer and nucleotide labelling to produce RPA-amplicons used for multiplex detection of antibiotic resistance genes

Direct labelling of amplification products using isothermal amplification is currently done most frequently by incorporating previously labelled primer. Although this method is well proven and widely used, it is not a universal solution due to some weaknesses. Alternatively, labelled nucleotides cou...

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Detalles Bibliográficos
Autores principales: Warmt, Christian, Broweleit, Lisa-Marie, Fenzel, Carolin Kornelia, Henkel, Jörg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514322/
https://www.ncbi.nlm.nih.gov/pubmed/37735542
http://dx.doi.org/10.1038/s41598-023-42830-7