High-Efficiency Transformation and Expression of Genomic Libraries in Yeast

Saccharomyces cerevisiae is a powerful system for the expression of genome-wide or combinatorial libraries for diverse types of screening. However, expressing large libraries in yeast requires high-efficiency transformation and controlled expression. Transformation of yeast using electroporation met...

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Detalles Bibliográficos
Autores principales: Loock, Mira, Antunes, Luiza Berenguer, Heslop, Rhiannon T, De Lauri, Antonio Alfonso, Brito Lira, Andressa, Cestari, Igor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514863/
https://www.ncbi.nlm.nih.gov/pubmed/37736972
http://dx.doi.org/10.3390/mps6050089

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514863/
https://www.ncbi.nlm.nih.gov/pubmed/37736972
http://dx.doi.org/10.3390/mps6050089

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