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High-Efficiency Transformation and Expression of Genomic Libraries in Yeast

Saccharomyces cerevisiae is a powerful system for the expression of genome-wide or combinatorial libraries for diverse types of screening. However, expressing large libraries in yeast requires high-efficiency transformation and controlled expression. Transformation of yeast using electroporation met...

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Detalles Bibliográficos
Autores principales:	Loock, Mira, Antunes, Luiza Berenguer, Heslop, Rhiannon T, De Lauri, Antonio Alfonso, Brito Lira, Andressa, Cestari, Igor
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514863/ https://www.ncbi.nlm.nih.gov/pubmed/37736972 http://dx.doi.org/10.3390/mps6050089

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