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Native ultrastructure of fresh human brain vitrified directly from autopsy revealed by cryo-electron tomography with cryo-plasma focused ion beam milling

Ultrastructure of human brain tissue has traditionally been examined using electron microscopy (EM) following chemical fixation, staining, and mechanical sectioning, which limit attainable resolution and introduce artifacts. Alternatively, cryo-electron tomography (cryo-ET) offers the potential to i...

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Detalles Bibliográficos
Autores principales: Creekmore, Benjamin C., Kixmoeller, Kathryn, Black, Ben E., Lee, Edward B., Chang, Yi-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516044/
https://www.ncbi.nlm.nih.gov/pubmed/37745569
http://dx.doi.org/10.1101/2023.09.13.557623