Cargando…

Native ultrastructure of fresh human brain vitrified directly from autopsy revealed by cryo-electron tomography with cryo-plasma focused ion beam milling

Ultrastructure of human brain tissue has traditionally been examined using electron microscopy (EM) following chemical fixation, staining, and mechanical sectioning, which limit attainable resolution and introduce artifacts. Alternatively, cryo-electron tomography (cryo-ET) offers the potential to i...

Descripción completa

Detalles Bibliográficos
Autores principales:	Creekmore, Benjamin C., Kixmoeller, Kathryn, Black, Ben E., Lee, Edward B., Chang, Yi-Wei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516044/ https://www.ncbi.nlm.nih.gov/pubmed/37745569 http://dx.doi.org/10.1101/2023.09.13.557623

Ejemplares similares

In situ Microfluidic Cryofixation for Cryo Focused Ion Beam Milling and Cryo Electron Tomography
por: Fuest, Marie, et al.
Publicado: (2019)

Skeletal Muscle Triad Junction Ultrastructure by Focused-Ion-Beam Milling of Muscle and Cryo-Electron Tomography
por: Wagenknecht, Terence, et al.
Publicado: (2015)

Fully automated, sequential focused ion beam milling for cryo-electron tomography
por: Zachs, Tobias, et al.
Publicado: (2020)

Comparison of the cryo-tolerance of vitrified gorgonian oocytes
por: Tsai, Sujune, et al.
Publicado: (2016)

CryoFIB milling large tissue samples for cryo-electron tomography
por: Wang, Sihan, et al.
Publicado: (2023)

Cryo-focused ion beam preparation of perovskite based solar cells for atom probe tomography
por: Rivas, Nicolás Alfonso, et al.
Publicado: (2020)

Cryo-electron Microscopy Specimen Preparation By Means Of a Focused Ion Beam
por: Rubino, Stefano, et al.
Publicado: (2014)

Integrated Fluorescence Microscopy (iFLM) for Cryo-FIB-milling and In-situ Cryo-ET
por: Yang, Jae, et al.
Publicado: (2023)

Protocol for live-cell fluorescence-guided cryoFIB-milling and electron cryo-tomography of virus-infected cells
por: Franken, Linda E., et al.
Publicado: (2022)

Ultrastructure of Exospore Formation in Streptomyces Revealed by Cryo-Electron Tomography
por: Sexton, Danielle L., et al.
Publicado: (2020)

Super-resolution confocal cryo-CLEM with cryo-FIB milling for in situ imaging of Deinococcus radiodurans
por: Sexton, Danielle L., et al.
Publicado: (2021)

High-resolution single-particle cryo-EM of samples vitrified in boiling nitrogen
por: Engstrom, Tyler, et al.
Publicado: (2021)

Ultrastructure of compacted DNA in cyanobacteria by high-voltage cryo-electron tomography
por: Murata, Kazuyoshi, et al.
Publicado: (2016)

Isolation, cryo-laser scanning confocal microscope imaging and cryo-FIB milling of mouse glutamatergic synaptosomes
por: Gogoi, Prerana, et al.
Publicado: (2022)

2.7 Å cryo-EM structure of vitrified M. musculus H-chain apoferritin from a compact 200 keV cryo-microscope
por: Hamdi, Farzad, et al.
Publicado: (2020)

Cryo-EM samples of gas-phase purified protein assemblies using native electrospray ion-beam deposition
por: Esser, Tim K., et al.
Publicado: (2022)

Cryo Electron Tomography of Native HIV-1 Budding Sites
por: Carlson, Lars-Anders, et al.
Publicado: (2010)

Cryo-electron microscopy of extracellular vesicles in fresh plasma
por: Yuana, Yuana, et al.
Publicado: (2013)

Cryo-EM structure of native human thyroglobulin
por: Adaixo, Ricardo, et al.
Publicado: (2022)

Quantification of gallium cryo-FIB milling damage in biological lamellae
por: Lucas, Bronwyn A., et al.
Publicado: (2023)

Heavy ion beam induced motion in rare gas cryo targets
por: Dornik, M, et al.
Publicado: (1995)

Resolving new ultrastructural features of cytokinetic abscission with soft-X-ray cryo-tomography
por: Sherman, Shachar, et al.
Publicado: (2016)

Quantifying organellar ultrastructure in cryo-electron tomography using a surface morphometrics pipeline
por: Barad, Benjamin A., et al.
Publicado: (2023)

Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography
por: Engel, Benjamin D, et al.
Publicado: (2015)

Ultrastructural comparison of dendritic spine morphology preserved with cryo and chemical fixation
por: Tamada, Hiromi, et al.
Publicado: (2020)

Cryo-milled nano-DAP for enhanced growth of monocot and dicot plants
por: Singh, Naorem Ronald Reagan, et al.
Publicado: (2021)

Cryo
Publicado: (1996)

Cryo-Electron Tomography Reveals the Complex Ultrastructural Organization of Multicellular Filamentous Chloroflexota (Chloroflexi) Bacteria
por: Gaisin, Vasil A., et al.
Publicado: (2020)

A versatile cryo-transfer system, connecting cryogenic focused ion beam sample preparation to atom probe microscopy
por: Macauley, Chandra, et al.
Publicado: (2021)

Correction: Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography
por: Engel, Benjamin D, et al.
Publicado: (2015)

Ultrastructural analysis of adult mouse neocortex comparing aldehyde perfusion with cryo fixation
por: Korogod, Natalya, et al.
Publicado: (2015)

Quantitative Cryo-Electron Tomography
por: Navarro, Paula P.
Publicado: (2022)

Structural, microstructural and magnetic evolution in cryo milled carbon doped MnAl
por: Fang, Hailiang, et al.
Publicado: (2018)

Structure of native chromatin fibres revealed by Cryo-ET in situ
por: Hou, Zhen, et al.
Publicado: (2023)

Cryo-soft X-ray tomography: using soft X-rays to explore the ultrastructure of whole cells
por: Harkiolaki, Maria, et al.
Publicado: (2018)

CryoETGAN: Cryo-Electron Tomography Image Synthesis via Unpaired Image Translation
por: Wu, Xindi, et al.
Publicado: (2022)

Cryo-soft X-ray tomography: a journey into the world of the native-state cell
por: Carzaniga, Raffaella, et al.
Publicado: (2013)

CRYO '90
por: Kelley, J Patrick
Publicado: (1991)

Efficient preparation of Arabidopsis pollen tubes for ultrastructural analysis using chemical and cryo-fixation
por: Ndinyanka Fabrice, Tohnyui, et al.
Publicado: (2017)

Simple method of thawing cryo-stored samples preserves ultrastructural features in electron microscopy
por: Galhuber, Markus, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_mrk6asqj9erla6o8kjoursij63