Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoprotein

Successful genome editing in primary human islets could reveal features of the genetic regulatory landscape underlying β cell function and diabetes risk. Here, we describe a CRISPR-based strategy to interrogate functions of predicted regulatory DNA elements using electroporation of a complex of Cas9...

Descripción completa

Detalles Bibliográficos
Autores principales: Bevacqua, Romina J., Zhao, Weichen, Merheb, Emilio, Kim, Seung Hyun, Marson, Alexander, Gloyn, Anna L., Kim, Seung K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516051/
https://www.ncbi.nlm.nih.gov/pubmed/37745551
http://dx.doi.org/10.1101/2023.09.16.558090

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516051/
https://www.ncbi.nlm.nih.gov/pubmed/37745551
http://dx.doi.org/10.1101/2023.09.16.558090

Ejemplares similares