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Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoprotein
Successful genome editing in primary human islets could reveal features of the genetic regulatory landscape underlying β cell function and diabetes risk. Here, we describe a CRISPR-based strategy to interrogate functions of predicted regulatory DNA elements using electroporation of a complex of Cas9...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Cold Spring Harbor Laboratory
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516051/ https://www.ncbi.nlm.nih.gov/pubmed/37745551 http://dx.doi.org/10.1101/2023.09.16.558090 |