Protocol for quantifying the in vivo rate of protein degradation in mice using a pulse-chase technique

The ability to measure the in vivo rate of protein degradation is a major limitation in numerous fields of biology. Here, we present a protocol for quantifying this rate in mice using a pulse-chase technique that utilizes an azide-bearing non-canonical amino acid called azidohomoalanine (AHA). We de...

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Detalles Bibliográficos
Autores principales: Hibbert, Jamie E., Jorgenson, Kent W., Zhu, Wenyuan G., Steinert, Nathaniel D., Hornberger, Troy A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10517276/
https://www.ncbi.nlm.nih.gov/pubmed/37729055
http://dx.doi.org/10.1016/j.xpro.2023.102574

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10517276/
https://www.ncbi.nlm.nih.gov/pubmed/37729055
http://dx.doi.org/10.1016/j.xpro.2023.102574

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