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Global DNA methylation and telomere length as markers of accelerated aging in people living with HIV and non-alcoholic fatty liver disease
Metabolic-dysfunction-associated fatty liver disease (MAFLD) is a comorbidity that generally increases in people living with HIV (PLWH). This condition is usually accompanied by persistent inflammation and premature immune system aging. In this prospective cohort study, we describe a straightforward...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10517540/ https://www.ncbi.nlm.nih.gov/pubmed/37741970 http://dx.doi.org/10.1186/s12864-023-09653-2 |
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author | Moreno, Elena Martínez-Sanz, Javier Martín-Mateos, Rosa Díaz-Álvarez, Jorge Serrano-Villar, Sergio Burgos-Santamaría, Diego Luna, Laura Vivancos, María Jesús Moreno-Zamora, Ana Pérez-Elías, María Jesús Moreno, Santiago Dronda, Fernando Montes, María Luisa Sánchez-Conde, Matilde |
author_facet | Moreno, Elena Martínez-Sanz, Javier Martín-Mateos, Rosa Díaz-Álvarez, Jorge Serrano-Villar, Sergio Burgos-Santamaría, Diego Luna, Laura Vivancos, María Jesús Moreno-Zamora, Ana Pérez-Elías, María Jesús Moreno, Santiago Dronda, Fernando Montes, María Luisa Sánchez-Conde, Matilde |
author_sort | Moreno, Elena |
collection | PubMed |
description | Metabolic-dysfunction-associated fatty liver disease (MAFLD) is a comorbidity that generally increases in people living with HIV (PLWH). This condition is usually accompanied by persistent inflammation and premature immune system aging. In this prospective cohort study, we describe a straightforward methodology for quantifying biomarkers of aging, such as DNA methylation and telomere length, in PLWH and in the context of another relevant condition, such as MAFLD. Fifty-seven samples in total, thirty-eight from PLWH and nineteen from non-PLWH participants with or without MAFLD, were obtained and subjected to DNA extraction from peripheral blood mononuclear cells (PBMCs). Global DNA methylation and telomere length quantification were performed using an adapted enzyme-linked immunosorbent assay (ELISA) and qPCR, respectively. The quantification results were analysed and corrected by clinically relevant variables in this context, such as age, sex, and metabolic syndrome. Our results show an increased association of these biomarkers in PLWH regardless of their MAFLD status. Thus, we propose including the quantification of these age-related factors in studies of comorbidities. This will allow a better understanding of the effect of comorbidities of HIV infection and MAFLD and prevent their effects in these populations in the future. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09653-2. |
format | Online Article Text |
id | pubmed-10517540 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-105175402023-09-24 Global DNA methylation and telomere length as markers of accelerated aging in people living with HIV and non-alcoholic fatty liver disease Moreno, Elena Martínez-Sanz, Javier Martín-Mateos, Rosa Díaz-Álvarez, Jorge Serrano-Villar, Sergio Burgos-Santamaría, Diego Luna, Laura Vivancos, María Jesús Moreno-Zamora, Ana Pérez-Elías, María Jesús Moreno, Santiago Dronda, Fernando Montes, María Luisa Sánchez-Conde, Matilde BMC Genomics Research Metabolic-dysfunction-associated fatty liver disease (MAFLD) is a comorbidity that generally increases in people living with HIV (PLWH). This condition is usually accompanied by persistent inflammation and premature immune system aging. In this prospective cohort study, we describe a straightforward methodology for quantifying biomarkers of aging, such as DNA methylation and telomere length, in PLWH and in the context of another relevant condition, such as MAFLD. Fifty-seven samples in total, thirty-eight from PLWH and nineteen from non-PLWH participants with or without MAFLD, were obtained and subjected to DNA extraction from peripheral blood mononuclear cells (PBMCs). Global DNA methylation and telomere length quantification were performed using an adapted enzyme-linked immunosorbent assay (ELISA) and qPCR, respectively. The quantification results were analysed and corrected by clinically relevant variables in this context, such as age, sex, and metabolic syndrome. Our results show an increased association of these biomarkers in PLWH regardless of their MAFLD status. Thus, we propose including the quantification of these age-related factors in studies of comorbidities. This will allow a better understanding of the effect of comorbidities of HIV infection and MAFLD and prevent their effects in these populations in the future. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09653-2. BioMed Central 2023-09-23 /pmc/articles/PMC10517540/ /pubmed/37741970 http://dx.doi.org/10.1186/s12864-023-09653-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Moreno, Elena Martínez-Sanz, Javier Martín-Mateos, Rosa Díaz-Álvarez, Jorge Serrano-Villar, Sergio Burgos-Santamaría, Diego Luna, Laura Vivancos, María Jesús Moreno-Zamora, Ana Pérez-Elías, María Jesús Moreno, Santiago Dronda, Fernando Montes, María Luisa Sánchez-Conde, Matilde Global DNA methylation and telomere length as markers of accelerated aging in people living with HIV and non-alcoholic fatty liver disease |
title | Global DNA methylation and telomere length as markers of accelerated aging in people living with HIV and non-alcoholic fatty liver disease |
title_full | Global DNA methylation and telomere length as markers of accelerated aging in people living with HIV and non-alcoholic fatty liver disease |
title_fullStr | Global DNA methylation and telomere length as markers of accelerated aging in people living with HIV and non-alcoholic fatty liver disease |
title_full_unstemmed | Global DNA methylation and telomere length as markers of accelerated aging in people living with HIV and non-alcoholic fatty liver disease |
title_short | Global DNA methylation and telomere length as markers of accelerated aging in people living with HIV and non-alcoholic fatty liver disease |
title_sort | global dna methylation and telomere length as markers of accelerated aging in people living with hiv and non-alcoholic fatty liver disease |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10517540/ https://www.ncbi.nlm.nih.gov/pubmed/37741970 http://dx.doi.org/10.1186/s12864-023-09653-2 |
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