Global DNA methylation and telomere length as markers of accelerated aging in people living with HIV and non-alcoholic fatty liver disease

Metabolic-dysfunction-associated fatty liver disease (MAFLD) is a comorbidity that generally increases in people living with HIV (PLWH). This condition is usually accompanied by persistent inflammation and premature immune system aging. In this prospective cohort study, we describe a straightforward...

Descripción completa

Detalles Bibliográficos
Autores principales: Moreno, Elena, Martínez-Sanz, Javier, Martín-Mateos, Rosa, Díaz-Álvarez, Jorge, Serrano-Villar, Sergio, Burgos-Santamaría, Diego, Luna, Laura, Vivancos, María Jesús, Moreno-Zamora, Ana, Pérez-Elías, María Jesús, Moreno, Santiago, Dronda, Fernando, Montes, María Luisa, Sánchez-Conde, Matilde
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10517540/
https://www.ncbi.nlm.nih.gov/pubmed/37741970
http://dx.doi.org/10.1186/s12864-023-09653-2
_version_ 1785109345454784512
author Moreno, Elena
Martínez-Sanz, Javier
Martín-Mateos, Rosa
Díaz-Álvarez, Jorge
Serrano-Villar, Sergio
Burgos-Santamaría, Diego
Luna, Laura
Vivancos, María Jesús
Moreno-Zamora, Ana
Pérez-Elías, María Jesús
Moreno, Santiago
Dronda, Fernando
Montes, María Luisa
Sánchez-Conde, Matilde
author_facet Moreno, Elena
Martínez-Sanz, Javier
Martín-Mateos, Rosa
Díaz-Álvarez, Jorge
Serrano-Villar, Sergio
Burgos-Santamaría, Diego
Luna, Laura
Vivancos, María Jesús
Moreno-Zamora, Ana
Pérez-Elías, María Jesús
Moreno, Santiago
Dronda, Fernando
Montes, María Luisa
Sánchez-Conde, Matilde
author_sort Moreno, Elena
collection PubMed
description Metabolic-dysfunction-associated fatty liver disease (MAFLD) is a comorbidity that generally increases in people living with HIV (PLWH). This condition is usually accompanied by persistent inflammation and premature immune system aging. In this prospective cohort study, we describe a straightforward methodology for quantifying biomarkers of aging, such as DNA methylation and telomere length, in PLWH and in the context of another relevant condition, such as MAFLD. Fifty-seven samples in total, thirty-eight from PLWH and nineteen from non-PLWH participants with or without MAFLD, were obtained and subjected to DNA extraction from peripheral blood mononuclear cells (PBMCs). Global DNA methylation and telomere length quantification were performed using an adapted enzyme-linked immunosorbent assay (ELISA) and qPCR, respectively. The quantification results were analysed and corrected by clinically relevant variables in this context, such as age, sex, and metabolic syndrome. Our results show an increased association of these biomarkers in PLWH regardless of their MAFLD status. Thus, we propose including the quantification of these age-related factors in studies of comorbidities. This will allow a better understanding of the effect of comorbidities of HIV infection and MAFLD and prevent their effects in these populations in the future. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09653-2.
format Online
Article
Text
id pubmed-10517540
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-105175402023-09-24 Global DNA methylation and telomere length as markers of accelerated aging in people living with HIV and non-alcoholic fatty liver disease Moreno, Elena Martínez-Sanz, Javier Martín-Mateos, Rosa Díaz-Álvarez, Jorge Serrano-Villar, Sergio Burgos-Santamaría, Diego Luna, Laura Vivancos, María Jesús Moreno-Zamora, Ana Pérez-Elías, María Jesús Moreno, Santiago Dronda, Fernando Montes, María Luisa Sánchez-Conde, Matilde BMC Genomics Research Metabolic-dysfunction-associated fatty liver disease (MAFLD) is a comorbidity that generally increases in people living with HIV (PLWH). This condition is usually accompanied by persistent inflammation and premature immune system aging. In this prospective cohort study, we describe a straightforward methodology for quantifying biomarkers of aging, such as DNA methylation and telomere length, in PLWH and in the context of another relevant condition, such as MAFLD. Fifty-seven samples in total, thirty-eight from PLWH and nineteen from non-PLWH participants with or without MAFLD, were obtained and subjected to DNA extraction from peripheral blood mononuclear cells (PBMCs). Global DNA methylation and telomere length quantification were performed using an adapted enzyme-linked immunosorbent assay (ELISA) and qPCR, respectively. The quantification results were analysed and corrected by clinically relevant variables in this context, such as age, sex, and metabolic syndrome. Our results show an increased association of these biomarkers in PLWH regardless of their MAFLD status. Thus, we propose including the quantification of these age-related factors in studies of comorbidities. This will allow a better understanding of the effect of comorbidities of HIV infection and MAFLD and prevent their effects in these populations in the future. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-023-09653-2. BioMed Central 2023-09-23 /pmc/articles/PMC10517540/ /pubmed/37741970 http://dx.doi.org/10.1186/s12864-023-09653-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Moreno, Elena
Martínez-Sanz, Javier
Martín-Mateos, Rosa
Díaz-Álvarez, Jorge
Serrano-Villar, Sergio
Burgos-Santamaría, Diego
Luna, Laura
Vivancos, María Jesús
Moreno-Zamora, Ana
Pérez-Elías, María Jesús
Moreno, Santiago
Dronda, Fernando
Montes, María Luisa
Sánchez-Conde, Matilde
Global DNA methylation and telomere length as markers of accelerated aging in people living with HIV and non-alcoholic fatty liver disease
title Global DNA methylation and telomere length as markers of accelerated aging in people living with HIV and non-alcoholic fatty liver disease
title_full Global DNA methylation and telomere length as markers of accelerated aging in people living with HIV and non-alcoholic fatty liver disease
title_fullStr Global DNA methylation and telomere length as markers of accelerated aging in people living with HIV and non-alcoholic fatty liver disease
title_full_unstemmed Global DNA methylation and telomere length as markers of accelerated aging in people living with HIV and non-alcoholic fatty liver disease
title_short Global DNA methylation and telomere length as markers of accelerated aging in people living with HIV and non-alcoholic fatty liver disease
title_sort global dna methylation and telomere length as markers of accelerated aging in people living with hiv and non-alcoholic fatty liver disease
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10517540/
https://www.ncbi.nlm.nih.gov/pubmed/37741970
http://dx.doi.org/10.1186/s12864-023-09653-2
work_keys_str_mv AT morenoelena globaldnamethylationandtelomerelengthasmarkersofacceleratedaginginpeoplelivingwithhivandnonalcoholicfattyliverdisease
AT martinezsanzjavier globaldnamethylationandtelomerelengthasmarkersofacceleratedaginginpeoplelivingwithhivandnonalcoholicfattyliverdisease
AT martinmateosrosa globaldnamethylationandtelomerelengthasmarkersofacceleratedaginginpeoplelivingwithhivandnonalcoholicfattyliverdisease
AT diazalvarezjorge globaldnamethylationandtelomerelengthasmarkersofacceleratedaginginpeoplelivingwithhivandnonalcoholicfattyliverdisease
AT serranovillarsergio globaldnamethylationandtelomerelengthasmarkersofacceleratedaginginpeoplelivingwithhivandnonalcoholicfattyliverdisease
AT burgossantamariadiego globaldnamethylationandtelomerelengthasmarkersofacceleratedaginginpeoplelivingwithhivandnonalcoholicfattyliverdisease
AT lunalaura globaldnamethylationandtelomerelengthasmarkersofacceleratedaginginpeoplelivingwithhivandnonalcoholicfattyliverdisease
AT vivancosmariajesus globaldnamethylationandtelomerelengthasmarkersofacceleratedaginginpeoplelivingwithhivandnonalcoholicfattyliverdisease
AT morenozamoraana globaldnamethylationandtelomerelengthasmarkersofacceleratedaginginpeoplelivingwithhivandnonalcoholicfattyliverdisease
AT perezeliasmariajesus globaldnamethylationandtelomerelengthasmarkersofacceleratedaginginpeoplelivingwithhivandnonalcoholicfattyliverdisease
AT morenosantiago globaldnamethylationandtelomerelengthasmarkersofacceleratedaginginpeoplelivingwithhivandnonalcoholicfattyliverdisease
AT drondafernando globaldnamethylationandtelomerelengthasmarkersofacceleratedaginginpeoplelivingwithhivandnonalcoholicfattyliverdisease
AT montesmarialuisa globaldnamethylationandtelomerelengthasmarkersofacceleratedaginginpeoplelivingwithhivandnonalcoholicfattyliverdisease
AT sanchezcondematilde globaldnamethylationandtelomerelengthasmarkersofacceleratedaginginpeoplelivingwithhivandnonalcoholicfattyliverdisease