A Post-translational Modification–enhanced Pull-down Method to Study Degron Domains and the Associated Protein Degradation Complexes

The identification and characterization of the ubiquitin E-ligase complexes involved in specific proteins’ degradation via the ubiquitin-proteasome system (UPS) can be challenging and require biochemical purification processes and in vitro reconstitution assays. Likewise, evaluating the effect of pa...

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Detalles Bibliográficos
Autores principales: Scalia, Pierluigi, Williams, Stephen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518775/
https://www.ncbi.nlm.nih.gov/pubmed/37753472
http://dx.doi.org/10.21769/BioProtoc.4816

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518775/
https://www.ncbi.nlm.nih.gov/pubmed/37753472
http://dx.doi.org/10.21769/BioProtoc.4816

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