Fluorescence in situ Localization of Pri-miRNAs in Isolated Arabidopsis thaliana Nuclei

Here, we present an approach combining fluorescence in situ hybridization (FISH) and immunolabeling for localization of pri-miRNAs in isolated nuclei of A. thaliana. The presented method utilizes specific DNA oligonucleotide probes, modified by addition of digoxigenin-labeled deoxynucleotides to its...

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Detalles Bibliográficos
Autores principales: Gulanicz, Tomasz, Zienkiewicz, Agnieszka, Zienkiewicz, Krzysztof, Kasprowicz-Maluski, Anna, Szweykowska-Kulinska, Zofia, Jarmolowski, Artur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518776/
https://www.ncbi.nlm.nih.gov/pubmed/37753471
http://dx.doi.org/10.21769/BioProtoc.4824

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518776/
https://www.ncbi.nlm.nih.gov/pubmed/37753471
http://dx.doi.org/10.21769/BioProtoc.4824

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