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Immunoprecipitation of Reporter Nascent Chains from Active Ribosomes to Study Translation Efficiency

The study of translation is important to the understanding of gene expression. While genome-wide measurements of translation efficiency (TE) rely upon ribosome profiling, classical approaches to address translation of individual genes of interest rely on biochemical methods, such as polysome fractio...

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Detalles Bibliográficos
Autores principales: Cacioppo, Roberta, Lindon, Catherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10518778/
https://www.ncbi.nlm.nih.gov/pubmed/37753466
http://dx.doi.org/10.21769/BioProtoc.4821